Preparation and formulation of HEPES buffer CAS NO.7365-45-9

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

【Name】2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

【CAS#】7365-45-9

【Molecular weight】238.305

【Molecular formula】C8H18N2O4S

【Molecular structure】

HEPES is a kind of nonionic buffer, the main component of which is hydroxyethyl piperazine ethylsulfonate. It has a good buffer capacity in the range of ph7.2-7.4. HEPES is widely used in cell culture medium, protein research, DNA / RNA extraction kit and PCR diagnostic kit.

HEPES as a buffer has a very good point, that is, it can maintain a relatively constant pH value in open culture or cell observation. In this kind of culture conditions, the cell culture apparatus needs to be completely closed to avoid a small amount of carbonate in the culture medium from scattering into the air. There are two ways to use HEPES buffer. The details are as follows:

1. HEPES can be directly added to the prepared culture medium according to the required concentration, and then filtrated and sterilized. 2.38g HEPES is added to every 1000ml culture medium. After dissolving, adjust the pH to 7.2 with LN NaOH, and use after filtrating and sterilized. At this time, the concentration of HEPES is 10 mmol / L

2. Before use, 99 ml of culture medium was added to 1 ml of storage medium, and the final concentration was still 10 mmol / L. Methods: 23.8g HEPES was dissolved in 90ml double distilled water, pH was adjusted to 7.5-8.0 with LN NaOH, then the volume was adjusted to 100ml with water, filtered and sterilized, and sub packed into small bottles (2ml / bottle), which need to be stored at 4 ℃ or - 20 ℃

In daily biochemical experiments, especially in the process of cell culture, HEPES buffer is usually indispensable. The use of HEPES buffer has been understood. Later, we will learn about the preparation method of HEPES.

The preparation method of HEPES: 1,2-dichloroethane was directly used as solvent, hydroxyethyl piperazine (5.00g, 0.02mol), potassium carbonate K2CO3 (6.00g, 0.04mol), 50ml 1,2-dichloroethane was added into a 100ml three mouth flask with mechanical stirring and thermometer, and the reaction time was 20h. Stop the reaction, filter and wash the filtered salt with 200 ml ethyl acetate (EA). The filtrate was dried to obtain 2.6 g HEPES solid.

In conclusion, HEPES buffer is widely used, and the method of use and preparation is not complicated. As a result, there are many HEPES businesses in the market, and there are only a few real domestic manufacturers at present. So at present, the market is a mixture of fish and eyes, and there are many middlemen who earn a price difference from it. Hubei new Desheng Material Co., Ltd. is a manufacturer recommended here. Desheng company has been specialized in the production and development of biological buffers for many years. It has multi-storey factory buildings and imported equipment, and has established a professional team responsible for R & D and production management. It has high production efficiency, excellent product quality, sufficient warehouse stock, and immediate delivery when placing an order. If you want to know more about Desheng products, you can contact customer service on the official website of Desheng for details.