How Acridine Ester Works in Chemiluminescence Immunoassays CAS NO.115853-74-2

1. As the manufacturer rather than trader, we are capable to provide more reasonable and competitive prices, flexible stock, strong control of manufacture process, therefore less batch difference and strong after-sale services;

2. Desheng is a manufacturer of a series of chemiluminescence immuniassay reagents, including lumino, isolumino, Acridinium Ester series and so on. We are capable to provide one-stop purchasing experience for you;

3. Desheng has been in this field for 14 years. Our products are self-developed and manufactured, besides, we have accumulated with a lot of clinical experience which enable strong problem solving capability for our end-users;

4. Desheng began to export since 2010, we are equipped with strong logistics capability which including packaging, selection of appropriate agent and forwarder,custom declaration and custom documentation preparation.

Chemiluminescence Techniques Chemiluminescence Immunoassay (CLIA) is an immunoassay technique that labels, the true "indicator" of the label's analytical reaction, is a luminescent molecule. The latest non-isotopic labeling applied to immunochemical techniques Nico is also the earliest, widely present in animals (such as fireflies) as a light-emitting system: bioluminescence. Acridine esters and acridine salts are commonly used chemiluminescent reagents and have a wide range of applications. Including acridinium ester DMAE-NHS, NSP-DMAE-NHS, acridinium salt NSP-SA, acridinium salt NSP-SA-NHS, acridinium hydrazide NSP-SA-ADH, acridinium ester ME-DMAE-NHS.
In general, luminescence is the emission of visible or near-visible light (k = 300–800 nm) radiation in the transition of electrons from an excited state to a ground state. Potential energy generated in atoms in the form of light. A spectrophotometric measure, luminescence has the advantage over absorbance that the former is an absolute measure, while the latter is relative. We make reference chemiluminescence because the type of luminescence is commonly used in immunoassay techniques to determine the most appropriate kinetic energy for chemical reactions capable of producing electronically excited states. In the direct chemiluminescence method, the label used for the luminophore is acridine, while the enzyme label used in the indirect method is the base adamantyl 1,2-dioxetane aryl phosphatase phosphate (AMPPD) Substrates and peroxidase luminol or its derivatives are used as substrates. In contrast to synthetic molecules such as AMPPD and isoluminol, derivatives of the base molecule are more stable to other luminescent labels and result in the characteristic quantum yield enhancement of luminescence. Activation of these substrates requires chemical or enzymatic reactions associated with immune responses. For example, the use of luminol and its derivatives for chemiluminescent labeling depends on enzymatically catalyzed immunoassay oxidase. The addition of enhancers (e.g. ferricyanide, metal ions) further promotes electron activation, ultimately resulting in an extremely high solubility sensitivity (mol-16 per liter) of ANA, certainly better than by other immunoassays. CLIA instruments steadily penetrate immunoassays
In the field of definitive analysis, it is ultimately used to measure serum hormones, drugs, vitamins, tumor concentration markers, infectious disease markers, myocardial injury markers and ultimately autoantibodies.
Desheng Biochemical, founded in 2005, is located in Wuhan, the hinterland of central China, with convenient transportation. As a manufacturer of chemiluminescence reagents, we supply luminol, luminol monosodium salt, isoluminol, acridine ester DMAE-NHS, NSP-DMAE-NHS, acridine salt NSP-SA, acridine salt NSP all year round -SA-NHS, acridinium hydrazide NSP-SA-ADH, acridinium ester ME-DMAE-NHS. welcome Call for inquiries