Preparation and storage of pipes buffer CAS NO.5625-37-6

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, which has a wide range of products and can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

4. Desheng has been engaged in foreign trade since 2010, and we have our own export right.

The Chinese name of PIPES is piperazine n, n 'di (2 ethanesulfonic acid). It belongs to the same family as HEPES buffer and is also a member of piperazine buffer family. It is an amphoteric buffer. The effective pH value of pipes ranges from 6.1 to 7.5 and will not complex with metals. Therefore, pipes is often used in solutions containing metal ions and can also be used to purify tubulin by cellulose phosphate chromatography, The recombinant GTP binding protein ARF1 and ARF2 were purified by gel filtration as a buffer to crystallize ketones from Escherichia coli and replace glyphosate buffers. In addition, pipes can form free radicals, so it is not suitable for redox system. In cation exchange chromatography, low concentration pipes buffer should be used because pipes has relatively large ionic strength and its pKa value is concentration dependent.

Pipes powder

Preparation method of pipes: if 1 liter of pipes buffer solution is to be prepared, 302g pipes powder shall be added to 600ml deionized water and adjusted to the required pH value with 10N sodium hydroxide.

Precautions for pipes buffer:

1. When preparing the solution for the experiment, it shall be prepared with deionized pure water. The container shall be cleaned more than 3 times before use. The solution with special requirements shall be tested for blank value first;

2. The prepared pipes solution shall be sealed and stored, and it can be prevented from decomposition by putting it in a dark brown container;

3. The skin shall not be in direct contact with the solution to avoid damage to the skin caused by toxic substances;

4. Avoid solution deterioration caused by drastic temperature change. It is recommended to store it at 4 ℃ to avoid analysis result error during use.

Hubei xindesheng materials is specialized in the research, development and production of biological buffers. It has been established for decades. Now, the biological buffers produced include Tris, HEPES, taps, mops, caps, bicine, EPPs, PEP and a series of biological buffers for customers to choose from. Price concessions, factory direct hair, so that you can easily buy affordable and high-quality products. High production efficiency, fast delivery efficiency, sufficient supply, buy and send. If you need anything, please feel free to contact us!