PIPES buffer Manufacturer CAS NO.5625-37-6

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, which has a wide range of products and can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

4. Desheng has been engaged in foreign trade since 2010, and we have our own export right.

PIPES, fully known as piperazine-1,4-diethylsulfonic acid, can maintain a basically constant pH value of the solution within a certain range. When a certain amount of acid and alkali is added to some solutions, it can hinder the change of pH of the solution, which is called buffer biological buffer. Such solutions are called buffer solutions. Pipes is an important member of good's buffer. Good's buffer is a kind of buffer system dedicated to life science research, which does not participate in and interfere with biochemical processes.

Structure diagram of piperazine-1,4-diethylsulfonic acid (pipes)

The pH buffer range of pipes is 6.1-7.5, insoluble in water and soluble in NaOH aqueous solution. Pipes is different from buffers containing di (2-hydroxyethyl) amino groups (such as bis Tris, bicine). It can not form stable complexes with most metal ions. It is suitable for buffers in solution systems containing metal ions. According to previous studies, PIPES can be used to purify tubulin by using cellulose phosphate chromatography, and to purify recombinant GTP binding protein ARF1 and ARF2 by gel filtration as a buffer to crystallize ketoenzyme from E. coli. Because pipes can form free radicals, it is not suitable for redox system. In cation exchange chromatography, low concentration pipes buffer should be used because pipes has relatively large ionic strength and its pKa value is concentration dependent.

Preparation method of pipes solutions with different pH values (about 1-2l)

1. Prepare 0.1M solution (a)

(1) NaOH / deionized water = 4G / 400ml... ①

（2）「①+PIPES 30.237g」…②

(3) ② + deionized water (about 600ml) = 1000ml

2. Prepare 0.1M NaOH solution (b): 4G NaOH / 1000ml deionized water

3.pH 5.6 1,000ml(A)+0ml(B) (A):(B)=5:0

pH 6.4 1,000ml(A)+200ml(B) (A):(B)=5:1

pH 6.8 1,000ml(A)+400ml(B) (A):(B)=5:2

pH 7.2 1,000ml(A)+600ml(B) (A):(B)=5:3

pH 7.7 1,000ml(A)+800ml(B) (A):(B)=5:4

Desheng has more than ten years of R & D and production experience in biological buffer. The pipes produced have high purity and good buffer capacity. In addition to pipes, biological buffers can also provide HEPES, Tris, mops and other high-quality raw materials, and have one-to-one procurement customer service to answer your questions. If you need, please call for details.