How to prepare PIPES buffer CAS NO.5625-37-6

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【Name】2,2'-piperazine-1,4-diylbisethanesulfonic acid

【CAS#】5625-37-6

【Molecular weight】302.368

【Molecular formula】C8H18N2O6S2

【Storage Conditions】Room temperature, light-proof and moisture-proof

【Molecular structure】

PIPES is a member of piperazine buffer family and belongs to the same family as HEPES. It is zwitterionic good's buffer and is effective in the pH range of 6.1 to 7.5. Because the pH range is slightly different from that of HEPES, the concentration of pipe is only half of that of HEPES. The buffer does not complex with metals, so it can be used in solutions containing metal ions. It can also be used for chromatographic analysis, immobilization of plant cells by electron microscopy, and as a substitute for glyphosate buffer.

The preparation steps of pipes are as follows

To prepare 1 liter of 1m pipes free acid buffer solution, 302.37g   Pipes free acid was added to 600 ml DH   two   O. Use 10N sodium hydroxide to adjust to the desired pH value. You can use a table in 1m pipes PDF protocol. Using DH   two   O fill to final volume 1L and sterilize with filter or autoclave. Stored in 4 ? C。 If you are using pipes sodium salt, please use 1m pipes Na PDF protocol instead.

matters needing attention:

When the pH value of the solution is above 6.5, the free acid of pipes is easy to dissolve, but the sodium salt of pipes is easy to dissolve. If you are using a buffer in the form of a free acid, use sodium hydroxide to convert it to a sodium salt to improve solubility at a lower pH. Like HEPES, pipe is not suitable for experiments involving redox reactions because it can lead to the formation of free radicals.