Characteristics and application of good's buffer pipes CAS NO.5625-37-6

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, which has a wide range of products and can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

4. Desheng has been engaged in foreign trade since 2010, and we have our own export right.

Pipes is a member of the series of ethanesulfonic acid buffers, with the Chinese name of 1-4-piperazine diethylsulfonic acid. It is one of the series of ethanesulfonic acid buffer reagents developed by good in 1960. It has some important characteristics common to good's buffer, such as medium pKa, maximum water solubility and minimum solubility in all other solvents, minimum salt effect, minimum PKA change force with temperature (pipes are insoluble in water) chemical and enzymatic stability, minimum absorption of visible or ultraviolet spectral range, easy synthesis, etc.

Pipes buffer

The effective buffer range of pipes is pH 6.1-7.5, PKA = 6.8 (25 ℃). Its PKA is close to the physiological pH and is suitable for cell culture. In addition, pipes can also be used as a buffer reagent in animal and plant histochemistry to minimize lipid damage and experimental illusion caused by glutaraldehyde fixation. It is worth noting that pipes is not suitable for redox because it can form free radicals.

Pipes can be used in cell culture medium in vitro. It was found that the temperature was 37 ℃ in the study of determining lactic acid in plasma by lactic acid oxidase method   Pipes can be stable for 172 hours. During immunofluorescence labeling, 4% paraformaldehyde prepared with 50mmol / L pipes buffer can be used for fixation. Pipes can meet the requirements of adenosine diphosphate (ADP) When combined with relaxed fan-shaped myofibroblasts, it needs very low conditions for complexing divalent cations. Pipes has a high-quality effective buffer range, and there are many other uses. As an important member of God's buffer family, it also has certain advantages in the field of biological buffer.

Desheng is a manufacturer specializing in the production of biological buffers and EDTA. At present, its biological buffer products include Tris, Tris-HCl, HEPES, taps, mops, caps, bicine, EPPs, PEP and a series of biological buffers. It has been established for more than ten years. It has rich R & D and production experience and product knowledge, and can provide customers with a lot of technical support and after-sales guarantee. If any If you need help, please feel free to contact us!