Application of pipes and Tricine buffer CAS NO.5625-37-6

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, which has a wide range of products and can provide you with more comprehensive services;

3. Desheng has been in this industry for 16 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

4. Desheng has been engaged in foreign trade since 2010, and we have our own export right.

What is pipes buffer

Pipesbuffer (cas5625-37-6), whose full chemical name is 1,4-piperazine diethylsulfonic acid, is a zwitterionic biological buffer. It is white powder solid at normal temperature. Pipesbuffer is insoluble in water (1g / L (100 ° C). It can be dissolved by mixing equimolar sodium salt solution and titrating to the appropriate pH value. For example, pipesdisodium saltbuffer (cas76836-02-7) and pipes hemisodium salt (cas100037-69-2).

Application of pipesbuffer

Pipesbuffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscope research. Pipesbuffer is similar to mesbuffer and mopsbuffer (also produced by Desheng). This good's buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non coordination buffer in metal ion solutions.

Advantages of pipesbuffer

According to the relevant literature, using the glutaraldehyde solution buffered by NaOH pipes in the ultrastructure of various tissues, such as human skin, human hypertrophic scar, mollusc neuron tissue, etc., the results show that more details between organelles and organelles can be observed. The cytoplasm is more uniform in consistency. Microfilaments and microtubules are more common in pipes tissues than NaOH pipes buffer. In addition, pipesbuffer has been shown to be comparable in living cells.

What is Tricine buffer

TRICINEBuffer(N-tris(Hydroxymethyl)methylglycine,CASNo. 5704-04-1), an amphoteric biological buffer containing amino and acid groups. This product is white crystalline powder, slightly soluble in water (solubility is 1.8g/100ml water).

Application of tricinebuffer

Tricine is widely used in cell culture and electrophoresis. As a buffer for cell culture research, Tricine can inhibit the accidental growth of Mycoplasma in animal tissue culture. It has also been used in bacterial culture media to prevent iron salt precipitation. The good's buffer can form complexes with most metal ions, such as Mg, CA, Co, Cu, Ni and Zn. Therefore, the stability constant and concentration should be considered when selecting the buffer. As a buffer for electrophoretic studies, Tricine has been used as a substitute for the protocol using the controlled substance barbital.

Advantages of Tricine buffer

Tricine has a higher negative (more negative) charge than glycine, which makes it migrate faster. In addition, its high ionic strength leads to more ionic movement and less protein movement, so that low molecular weight proteins can be separated in a lower percentage of acrylamide gel. Tricine has been shown to be useful for the separation of proteins in the range of 1 to 100 kDa by electrophoresis. In ten tests using firefly luciferase for ATP determination, it was found that 25 mmol / L Tricine buffer was Zui effective buffer.