Application of mops buffer and precautions CAS NO.1132-61-2

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

The Chinese name of mops buffer is 3 - (n-morpholinyl) propanesulfonic acid, which belongs to zwitterionic buffer. It has white powder appearance, high hydrophilicity and excellent water solubility (1000g / L at 20 ℃). The appearance of dissolved mops aqueous solution is colorless. Mops at 25 ° The pKa value was 7.14 at 0.5 C, and the effective pH range was 6.5-7.9. Therefore, mops is often used in molecular biology and biochemical research.

Mops powder

The main application fields of mops as buffer are as follows:

(1) In vitro diagnosis: biochemical kit, DNA / RNA extraction kit and PCR kit.

(2) protein / nucleic acid isolation and purification: separation in nucleic acid and protein electrophoresis, denaturing gel electrophoresis of RNA1, and protein purification in chromatography.

(3) Cell culture medium: buffer system of culture medium.

(4) Biosynthesis: buffer used in biosynthesis.

(5) The absorption was measured by ultraviolet / visible spectrophotometry and the redox characteristics were studied by cyclic voltammetry.

(6) Under the control of medium pH   5, including cell culture media for yeast, bacteria and mammalian cells.

Problems to be paid attention to when using mops buffer solution:

1. When mops buffer was used in mammalian cell culture, the concentration of mops could not be higher than 20 mm;

2. There is almost no complexation between mops and metal, but once there is complexation, there may be interference;

3. Mops can affect the thickness of endothelial surface layer and barrier performance;

4. In the presence of glucose, mops was partially degraded during autoclaving.

5. Mops interact with DNA and form complex;

6. Mops can change the interaction of lipids;

Hubei xindesheng Materials Co., Ltd. has been specialized in the research and development of biological buffers for decades, and now produces a series of biological buffers such as mops, Tris, HEPES, taps, caps, bicine, EPPs, PEP for customers to choose. Price concessions, manufacturers direct, so that you can easily buy affordable quality products. High production efficiency, fast delivery efficiency, sufficient supply, immediate delivery. If necessary, please feel free to contact us!