Preferential price of pipes buffer manufacturers CAS NO.5625-37-6

1.Factory outlets, independent research and development production, quality assurance, high cost performance;

2. Purity≥99%, the reagent has good water solubility, stable process and low concentration of pipes buffer is suitable for cation exchange chromatography.

3.Desheng is a professional manufacturer of biological buffers. Its products mainly include Tris, Tris HCl, bicine, caps, mops, taps, EPPs, HEPES, PEP, pipes, MOPSO, etc. We are capable to provide you with a one-stop purchasing experience.

4.As a manufacturer rather than a trader, we have the ability to offer more reasonable and competitive prices. We have flexible inventory, professional production team, strict quality testing, high-quality after-sales service, no difference between batches;

5.Desheng has 14 years of production and R & D experience in this field. All our products are independently developed and produced, and have accumulated rich clinical experience, which can provide our end users with strong problem-solving ability;

6.We have excellent logistics services, professional packaging services, rich export experience, rich experience in loading and unloading a large number of containers in Chinese ports, and fast shipping by famous shipping companies.

Product information：　

　　【English name】2,2'-piperazine-1,4-diylbisethanesulfonic acid

       【Abbreviation】PIPES

　　【CAS#】5625-37-6

　　【Molecular weight】302.368

　　【Molecular formula】C8H18N2O6S2

　　【Storage condition】away from light and moisture at room temperature

       PIPES is a kind of substance which can keep the pH value of solution constant in a certain range. When a certain amount of acid and alkali is added to some solutions, it will hinder the pH change of the solution, which is called buffering biological buffer. Such a solution is called buffer solution. Pipes is an important member of good's buffer. Good's buffer is a kind of buffer system specially used in life science research, which does not participate in or interfere with biochemical processes.

Structure diagram of piperazine-1,4-diethylsulfonic acid (pipes)

The pH buffer range of pipes is 6.1-7.5, insoluble in water and soluble in NaOH solution. Different from buffers containing bis (2-hydroxyethyl) amino groups (such as bis Tris, bicine), pipes can not form stable complexes with most metal ions, so it is suitable for buffers containing metal ions. According to previous studies, PIPES can be used to purify tubulin by using cellulose phosphate chromatography, and to purify recombinant GTP binding protein ARF1 and ARF2 by gel filtration as a buffer to crystallize ketoenzyme from E. coli. Due to the formation of free radicals, pipe is not suitable for redox system. In cation exchange chromatography, low concentration of pipes buffer should be used because of its relatively high ionic strength and concentration dependent pKa value.

Preparation method of different pH values of pipes solution (about 1-2l)

1. Prepare 0.1M solution (a)

(1) NaOH / deionized water = 4G / 400ml... ①

（2）「①+PIPES 30.237g」…②

(3) ② + deionized water (about 600ml) = 1000ml

2. Prepare 0.1M NaOH solution (b): NaOH 4G / deionized water 1000ml

3.pH 5.6 1,000ml(A)+0ml(B) (A):(B)=5:0

pH 6.4 1,000ml(A)+200ml(B) (A):(B)=5:1

pH 6.8 1,000ml(A)+400ml(B) (A):(B)=5:2

pH 7.2 1,000ml(A)+600ml(B) (A):(B)=5:3

pH 7.7 1,000ml(A)+800ml(B) (A):(B)=5:4

Desheng has more than ten years of experience in R & D and production of biological buffers. The produced pipes have high purity and good buffering capacity. In addition to pipes, biological buffers can also provide HEPES, Tris, mops and other high-quality raw materials, and have one-to-one purchasing customer service to answer your questions, if you need, please call for details.