Application and precautions of 4-Morpholinepropanesulfonic Acid CAS NO.1132-61-2

1. Factory production, more preferential prices, regular products available in stock;

2. Desheng company is an enterprise that produces biological buffer products, with many products, which can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. The purity of biological buffer is high and the product quality is guaranteed.

4.Desheng has been doing foreign trade since 2010. We have our own export right.

Chinese name of MOPS buffer is 3-(N-morpholinyl)propanesulfonic acid, which is a zwitterionic buffer with a white powder appearance, high hydrophilicity and excellent water solubility (1000g/L at 20°C) . The appearance of dissolved MOPS in its aqueous solution is also colorless. The pKa value of MOPS at 25°C is 7.14, and the effective pH adjustment range is 6.5-7.9. Therefore, MOPS is often used in molecular biology and biochemical research.
The main application areas of MOPS as a buffer are as follows:
(1) In vitro diagnosis: biochemical kits, DNA/RNA extraction kits and PCR kits.
(2) Protein/nucleic acid separation and purification: separation in nucleic acid and protein electrophoresis, denaturing gel electrophoresis of RNA1, and protein purification in chromatography.
(3) Cell culture medium: the buffer system of the medium.
(4) Biosynthesis: Buffer used in biosynthesis.
(5) Measure absorption in UV/visible spectrophotometry and study redox properties using cyclic voltammetry.
(6) Including cell culture media for yeast, bacteria and mammalian cells when the pH of the medium is controlled at 5.
Matters needing attention when using MOPS buffer:
1. When mops buffer is used for mammalian cell culture, the concentration of MOPS should not be higher than 20mm;
2. There is almost no complexation between MOPS and metal, but once complexation occurs, interference may occur;
3. MOPS can affect the thickness and barrier properties of rat endothelial surface layer;
4. In the presence of glucose, MOPS is partially degraded during autoclaving.
5. MOPS It interacts with DNA and forms complexes;
6. MOPS can alter lipid interactions;
Hubei Xindesheng Materials specializes in the R&D and production of biological buffers. It has been established for decades. The biological buffer products it currently produces include a series of biological buffers such as MOPS, TRIS, HEPES, TAPS, CAPS, BICINE, EPPS, and PEP. Available for customers to choose from. Price concessions, factory direct, allowing you to easily buy affordable and high-quality products. High production efficiency, fast delivery efficiency, sufficient supply, and immediate delivery. Feel free to contact us if necessary!