3-Morpholinepropanesulfonic acid MOPS Product Details CAS NO.1132-61-2

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The full chemical name of MOPS is 3-morpholinepropanesulfonic acid. It is a white crystalline powder at room temperature with good hydrophilicity. 10g of MOPS powder can be completely dissolved in 100ml of water at 20°C, and the dissolved solution is colorless and transparent. MOPS is often prepared as a biological buffer to adjust the pH of the solution in biochemical reactions, and is a zwitterionic biological buffer.
The basic information of Mops is as follows:
CAS number: 1132-61-2
Molecular formula: C7H15NO4S
Molecular weight: 209.3
Applicable PH range: 6.5-7.9
PKA value (25°C): 7.0-7.4
Purity: 99%
Absorbance: less than 0.05
As a common buffer, MOPS buffer has a wide range of applications. The editor of Desheng will explain MOPS in detail below.
Several applications of buffers:
1. Since the pH range is between 6.5-7.9, MOPS buffer can be used to separate, purify, and extract RNA and protein, but cannot be used to separate DNA because it can interact with DNA and form complexes;
2. MOPS buffer can combine with iron, but does not react with most metals such as magnesium, manganese, cobalt, nickel, etc. It is often added to cell culture media that do not require iron ions, such as bacteria, charcoal yeast, and mammalian cells. MOPS buffer to stabilize the acidity of the solution. It should be noted that the concentration of the buffer has a great influence on the pH of the solution, so when using MOPS
When the buffer adjusts the pH value of the cell culture, it should be noted that the concentration of MOPS should be less than 20mM.
3. In the non-denaturing agarose gel electrophoresis experiment of RNA, MOPS buffer is more suitable for stabilizing the pH value of the solution than other buffers.
4. Because the UV absorbance of MOPS buffer is very small, it does not interfere with the measurement of absorbance during UV/Vis spectrophotometry,
5. At room temperature, the chemical properties of MOPS buffer are very stable. When it interacts with the peptide backbone of bovine serum albumin, it can promote the protein to reach a static stable state. MOPS buffer has a strong buffering capacity in the pH range of 6.5-7.9. Note that it can change
The interaction between lipids, such as affecting the thickness and barrier properties of the rat endothelial surface, the mRNA expression form of bovine embryos produced in vitro, etc., will cause oxidation and other reactions when encountering strong oxidants such as strong acids and alkalis. Therefore, in the application, to avoid these problems.
Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffers. The process is stable and can ensure that the appearance of the product is pure white crystal powder. Welcome friends to come to consult. Welcome friends to come to consult.