The action of 3 - ((1,3-dihydroxy-2 - (hydroxymethyl) propane-2-yl) amino) propane-1-sulfonic acid CAS NO.29915-38-6

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Product information:

[Name] 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid

[CAS No.] 29915-38-6

[Molecular weight] 243.28

[Molecular formula] C7H17NO6S

[Storage conditions] at room temperature, away from light and moisture

[chemical structure] 

Taps, full name n-tri (hydroxymethyl) methyl-3-aminopropanesulfonic acid, is a zwitterionic buffer, widely used in the field of Biochemistry and molecular biology, CAS No.: 29915-38-6, pH buffer range 7.7-9.1, soluble in water (25g / 50ml). The following is a brief introduction to the synthesis, application and instructions of taps by Desheng editor. I hope you can have a more comprehensive understanding of taps after reading this article.

Synthesis of biological buffer taps

In this paper, a synthesis process of taps is introduced. Tris and 1,3-propanesulfonic acid lactone (1,3-ps) are used as raw materials to react in alcohol solvent

1. Weigh equal moles of Tris and 1,3-ps, and take appropriate amount of alcohol solvent into a 250ml three port flask with condensation reflux. Under the condition of stirring, heat up and reflux;

2. Stop the reaction after a period of time; At room temperature, after the reaction solution is cooled to room temperature, the tap filter cake is washed 2 ~ 3 times with an appropriate amount of anhydrous ethanol, then the tap filter cake is taken out and dried in a vacuum drying oven, and the filtrate is recovered for use.

Application of biological buffer taps

1. Taps is a common buffer system in DNA screening system, and it can also be used as a buffer component of RNA sample, which is suitable for electron transfer and phosphorylation of chloroplast thin layer preparation

2. Protect oxyhemoglobin from oxidation to methemoglobin during freeze-drying

3. It can be used as a background electrolyte for protein microanalysis by capillary zone electrophoresis.

4. As an important pH stabilizer, it can be mixed with weak acid and its conjugated base to obtain proper pH value.

5. It is usually used in some biochemical diagnostic kits, PCR diagnostic kits and DNA / RNA extraction kits