How to make N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic Acid buffer CAS NO.29915-38-6

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Product information:

[Name] 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid

[CAS No.] 29915-38-6

[Molecular weight] 243.28

[Molecular formula] C7H17NO6S

[Storage conditions] at room temperature, away from light and moisture

Taps full name: trimethylolmethylamino propane sulfonic acid, belongs to a kind of biological buffer. The appearance is white crystal powder, which volatilizes in the air at room temperature and has a pungent smell. It has good water solubility, with an average of 50g taps per 100g water. It can also be dissolved in na0h lye, and the water solution is colorless and transparent. CAS number is 29915-38-6, molecular formula is c7h17n06s, molecular weight is 243.28. Taps is also a kind of amphoteric ion buffer widely used in biochemistry and molecular biology, mainly used in biochemical diagnostic kits, DNA / RNA extraction kits and PCR diagnostic kits.

At present, there are few reports about the synthesis process of taps. The synthesis method of taps was studied in detail according to the information on the Internet. The results showed that trimethylol aminomethane and 1,3-propanesulfonic acid lactone were used as raw materials to react in alcohol solvent.

Specific operation: weigh equal molar amount of Tris and 1,3-ps, and take appropriate amount of alcohol solvent into a 250ml three port flask with condensation reflux. Under the condition of stirring, heat up and reflux; Stop the reaction after a period of time; At room temperature, after the reaction solution is cooled to room temperature, the tap filter cake is washed 2 ~ 3 times with an appropriate amount of anhydrous ethanol, then the tap filter cake is taken out and dried in a vacuum drying oven, and the filtrate is recovered for use. In the above synthesis process, the reaction time, different alcohol solvents and the recycling of reaction solvents have some effects on the yield and purity of taps. The results showed that the yield was the highest when the reaction time was controlled at 6 hours, and the yield decreased slightly when the reaction time was prolonged; For ethanol, n-propanol, isopropanol and n-butanol, n-butanol was the best solvent for taps; In the reaction process, the recycling of reaction solvent is conducive to improve the yield and purity of taps. In conclusion, taps was synthesized from Tris and 1,3-ps, the reaction time was controlled at 6 hours, n-butanol was used as solvent, and recycled in the reaction process, the yield and purity of taps were the best.

Hubei xindesheng Materials Co., Ltd. specializes in the production and R & D of biological buffer, enzyme preparation, blood collection additive, Carbomer, chemiluminescence reagent and chromogen substrate. At present, Desheng company has been established for decades, has its own R & D team, and has invested a lot of energy in biological buffers. At present, it has produced Tris, taps, bicine, caps, mops, MOPSO, EPPs, HEPES, pipes, PEP and other biological buffers. Reliable product quality, price concessions can be discussed, interested in understanding, you can call consultation, Desheng welcome your call.