Application of taps in biological research CAS NO.29915-38-6

1. Our products are independent research and production, quality assurance, cost-effective

2. Chloride ion content < 0.01%, purity ≥ 99%, good water solubility and stable process

3. Desheng is an enterprise specializing in the production of biological buffer. We have the ability to provide you with one-stop purchasing experience.

4. As a manufacturer rather than a trader, we have the ability to provide more reasonable and competitive prices, sufficient inventory, professional production team, strict quality inspection, strong after-sales service, and no difference between batches;

5. Desheng has 14 years of production and R & D experience in this field. All our products are independently developed and produced. We have accumulated rich clinical experience and provided our customers with strong problem-solving ability

6. With strong logistics ability, professional packaging service and rich export experience, our company has rich experience in loading and unloading a large number of containers in Chinese ports, and has the fast shipping experience of famous shipping companies

The biological buffer Taps   can be used as a buffer for nucleic acid DNA or RNA in capillary zone electrophoresis. Capillary zone electrophoresis is different from electrophoresis in electrophoresis tank. Electrophoresis in electrophoretic tank usually requires separation of DNA or nucleic acid from agarose gel or other gel gels, and separation of various particles can be carried out directly in capillary tubes.

The inner diameter of the capillary used for electrophoresis is less than 100 μ M (usually 50 ~ 75) μ m) An elastic quartz capillary with a length of 30 ~ 100cm is generally used to analyze charged solutes. In order to reduce the phenomenon of electroosmosis and adsorption, the inner wall of the capillary can be coated.

Capillary zone electrophoresis is an electrophoretic separation and analysis method, which takes elastic quartz capillary as the separation channel and high-voltage DC electric field as the driving force, and realizes the separation according to the differences in mobility and distribution behavior of each component in the sample.

Biological buffer taps reagent powder

When the pH value of the quartz capillary column used for thin tube electrophoresis is > 3, its inner surface is negatively charged, and an electric double layer is formed when it is in contact with the buffer. Under the action of high voltage electric field, the buffer on one side of the electric double layer moves to the negative direction due to positive charge, thus forming electroosmotic flow.

At the same time, in the buffer solution, the charged particles move to the opposite direction of the charge polarity at different speeds under the action of electric field to form electrophoresis.

The migration velocity of charged particles in capillary buffer is equal to the vector sum of electrophoresis and electroosmosis. Various particles are separated due to different migration speeds caused by different charge, mass, volume and shape.

The stationary phase of HPLC can also be filled into the capillary or coated on the inner wall of the capillary to form capillary electrochromatography. This mode has the separation mechanism of electrophoresis and liquid chromatography.

During capillary electrophoresis, the tube needs to be filled with buffer to ensure operation. Organic solvent modification and surfactant can also be added. Degassing is required before the operation buffer is used. Taps buffer is very suitable for nucleic acid experiment, so taps buffer is very suitable for capillary zone electrophoresis buffer of DNA or RNA.

The reagent for preparing taps buffer is synthesized by trimethylolaminomethane tham and 1,3 propanesulfonic acid lactone in alcohol solvent. Desheng also produces tham buffer. Therefore, it has technical and cost advantages in the synthesis of taps and is a recommended biological buffer manufacturer.