Application of bicine buffer CAS NO.150-25-4

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Biological buffer is an organic substance with neutralization effect of hydrogen ion on the surface. The biological buffer helps to maintain the correct temperature

So that the biochemical process can continue to operate optimally. Most buffers are composed of weak acids and bases. Even if acid or alkali is added,

They also help to maintain a given pH. As an important member of the biological buffer family, what is bicine superior to

1) What is bicine biological buffer

Bicine buffer (n-tris (hydroxymethyl) methylglycine, CAS: CAS: 150-25-4), bicine is

An organic compound used as a buffer. It's one of the good's buffers, at 20 ° The pKa at C is 8.35. It's through

Glycine reacted with ethylene oxide and then hydrolyzed to obtain lactone. Bicine was synthesized by the addition of O2, H2S or thiosulfate

In the presence of amine. It is a kind of zwitterionic biological buffer containing amine and acid group. Properties white crystalline powder,

The solubility of bicine is 1.8 g / 100 ml water

2) Application of bicine buffer

Bicine is widely used in cell culture and electrophoresis. As a buffer for cell culture, bicine has inhibitory effect

Objective to investigate the ability of Mycoplasma to grow unexpectedly in animal tissue culture. It has also been used in bacterial culture media to prevent precipitation of iron salts. The

Good's buffer has the ability to form complexes with most metal ions, so stability should be considered when selecting the buffer

Qualitative basis and viscosity. As a buffer for electrophoretic studies, bicine has been used to develop a controlled substance barbital

It's a good alternative.

3) Advantages of biological buffer bicine

Bicine can combine with hydroxyl radical and form formaldehyde in the reaction process. In the presence of enzymes with oxidase activity

When bicine is used at room temperature, it may form triazine nitrogen oxide radicals. Compared with other buffers, bicine buffer was more stable

The yield of small peptides was higher when glycine was separated from the solution. In addition, bicine can produce more light in the presence of ATP and luciferase.

4) Why choose bicine of Desheng company

As a professional manufacturer of biological buffer, Desheng biochemical provides high-quality bicine according to your application, with purity (drop)

Calibration: ≥ 99%. Useful pH range: 7.4 – 8.8, often used as a buffer in biological and biochemical research

The granules are zwitterionic. Biological buffer bicine also provides special testing services, such as bioloading, heavy metals and proteins

Enzyme activity test, etc. In addition, we also provide a variety of packaging options. Desheng biochemical is absolutely the best choice for you to buy bicine

Choose!