Production method of mops buffer CAS NO.1132-61-2

1. Factory production, more preferential prices, regular products available in stock;

2. Desheng company is an enterprise that produces biological buffer products, with many products, which can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. The purity of biological buffer is high and the product quality is guaranteed.

4.Desheng has been doing foreign trade since 2010. We have our own export right.

Mops is a widely used and very important buffer. Mops itself is a good's buffer, which can be used not only as a buffer system for cell culture medium, but also as a common electrophoresis buffer and protein purification chromatographic buffer. In addition, mops is also used in biochemical diagnostic kits, such as DNA / RNA extraction kit, PCR kit, creatinine determination kit, etc.

At present, the production method of mops usually needs to use organic solvents, which not only increases the production cost, but also has an impact on the health of operators, and the recovery of organic solvents is also more troublesome. In this paper, we will introduce a method to prepare mops without using organic solvent. This method uses morpholine and propanesulfonic lactone as raw materials and is completed in six steps. It can not only save cost, reduce pollution, but also avoid safety risks. The specific steps are as follows:

1: First, morpholine was dissolved in water;

2: 1,3-propanesulfonic acid lactone was dripped into morpholine aqueous solution, then morpholine and 1,3-propanesulfonic acid lactone were subjected to ring opening addition reaction at 25 ℃ for 4-10 hours to form 3-morpholine propanesulfonic acid aqueous solution;

3: The water and residual morpholine were removed by vacuum dehydration of 3-morpholine propanesulfonic acid aqueous solution. Then the system was cooled to 5 ℃ for crystallization to obtain crude 3-morpholine propanesulfonic acid;

4: The crude 3-morpholine propanesulfonic acid was recrystallized;

5: The recrystallized crystal was filtered and put into a centrifuge for centrifugal dehydration. The mother liquor was concentrated, cooled, crystallized and washed with absolute ethanol to synthesize 3-morpholine propanesulfonic acid.

6: The dehydrated 3-morpholine propanesulfonic acid was washed with anhydrous ethanol, then filtered and centrifuged to remove the solvent, and then dried to obtain 3-morpholine propanesulfonic acid.

The preparation method of mops uses water as solvent, and no organic solvent is used in the process. At the same time, the production cost is reduced, the risk of harm to the health of operators is reduced, and there is no trouble of recycling organic solvents, and there is no problem of environmental pollution.

Desheng biology has been developing and producing mops for many years, and has invested a lot of energy in it. Now it has rich experience in the production of mops. The mops produced by Desheng has high purity, good water solubility, stable production and strict quality management system. At present, the company's products have been sold to many countries around the world, most of them are well received and re purchased, which has become a hot demand. If you are interested in understanding, you can call to consult, product quality, price can be discussed in detail, Desheng welcome your call.