Application and precautions of mops buffer CAS NO.1132-61-2

1. Factory production, more preferential prices, regular products available in stock;

2. Desheng company is an enterprise that produces biological buffer products, with many products, which can provide you with more comprehensive services;

3. Desheng has been in this industry for 14 years. Our products are independently developed and manufactured. The purity of biological buffer is high and the product quality is guaranteed.

4.Desheng has been doing foreign trade since 2010. We have our own export right.

The Chinese name of Mops buffer is 3 - (n-morpholinyl) propanesulfonic acid. It belongs to zwitterionic buffer. It appears as white powder, with high hydrophilicity and excellent water solubility (1000g / L at 20 ℃). The appearance of aqueous solution of dissolved mops is also colorless. The pKa value of mops at 25 ° C is 7.14, and the effective pH range is 6.5-7.9. Therefore, mops is often used in molecular biology and biochemical research.

Mops powder

The main application fields of mops as buffer are as follows:

(1) In vitro diagnosis: biochemical kit, DNA / RNA extraction kit and PCR kit.

(2) protein / nucleic acid isolation and purification: separation in nucleic acid and protein electrophoresis, denaturing gel electrophoresis of RNA1, and protein purification in chromatography.

(3) Cell culture medium: buffer system of culture medium.

(4) Biosynthesis: buffer used in biosynthesis.

(5) Absorption was measured in ultraviolet / visible spectrophotometry and redox characteristics were studied by cyclic voltammetry.

(6) Under control of medium pH   5, including cell culture medium for yeast, bacteria and mammalian cells.

Problems needing attention when using mops buffer:

1. When mops buffer is used for mammalian cell culture, the concentration of mops should not be higher than 20mm;

2. There is almost no complexation between mops and metal, but once complexation occurs, it may produce interference;

3. Mops can affect the thickness and barrier performance of rat endothelial surface layer;

4. In the presence of glucose, mops will be partially degraded during autoclaving.

5. Mops, which interacts with DNA and forms complexes;

6. Mops can change the interaction of lipids;

Hubei xindesheng materials is specialized in the research, development and production of biological buffers. It has been established for decades. Now, the biological buffers produced include mops, Tris, HEPES, taps, caps, bicine, EPPs, PEP and a series of biological buffers for customers to choose from. Price concessions, factory direct hair, so that you can easily buy affordable and high-quality products. High production efficiency, fast delivery efficiency, sufficient supply, buy and send. If you need anything, please feel free to contact us!