Chemiluminescence reagent acridine ester labeling CAS NO.194357-64-7

1. Factory direct sales, independent R & D and production, quality assurance and high cost performance;

2. It has the advantages of high sensitivity, good water solubility, stable process, high cost performance and small batch difference. It can be used for chemiluminescence and immunoassay. It has the advantages of less initiator, high sensitivity and simple operation.

3. Desheng is a professional manufacturer of chemiluminescence immunoassay reagents. Its main products include luminol, isoluminol, acridine ester series, etc. we have the ability to provide you with a one-stop purchasing experience.

4. As a manufacturer rather than a trader, we have the ability to provide more reasonable and competitive prices. We have flexible inventory, professional production team, strict quality inspection, high-quality after-sales service and no batch difference;

5. Desheng has 16 years of production and R & D experience in this field. All our products are independently developed and produced, have accumulated rich clinical experience, and can provide our end users with strong problem-solving ability;

6. We have excellent logistics services, professional packaging services, rich export experience, rich experience in loading and unloading a large number of containers in Chinese ports, and fast shipping of famous shipping companies.

Labeling principle of chemiluminescence substrate acridine ester in diagnostic reagent in chemiluminescence immunoassay, we use acridine ester to label the antibody protein first, and then we can detect the substance to be tested after the immune reaction. Therefore, how to label the antibody is very key. Acridine salts and related compounds have been widely proved to be very useful chemiluminescence markers. Their stability, labeling specificity and detection sensitivity are better than radioisotopes.

Acridine NHS ester, namely acridine succinimide ester (provided by this kit), can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as a leaving group, and protein forms a stable amide bond with acridine ester. After the reaction is completed, the excess acridine salt is removed through the desalination column. In the presence of alkaline hydrogen peroxide, acridine labeled proteins can emit light without enzyme catalysis. Therefore, the addition of excitation solution causes the reaction system to immediately release about 430nm photons. The concentration of protein can be detected by counting the number of photons with a standard photometer luminometer. Because the luminescence process is very short (the whole process is completed in 2 seconds), the sample must be placed directly in front of the photon detector inside the photometer. Proteins, peptides, antibodies and nucleic acids can be labeled with acridine ester.

Chemiluminescence reagent it should be noted here that when dissolving acridine succinimide ester, the DMF used must be strictly anhydrous to prevent the hydrolysis of acridine ester. When not in use, it should be dried and sealed at - 20 ° C for storage. Aiming at the traditional acridine ester, its structure was modified to increase the steric hindrance and enhance the hydrolysis resistance. For example, it is stable in Pb buffer with pH 7.0 at room temperature. If the acridine carboxylic acid does not contain - NHS, it needs to add condensing agent EDCI, etc. in order to have coupling reaction with the protein containing amino group. Acridine hydrazide contains free amino groups. The terminal of acridine hydrazide is suitable for direct coupling of polysaccharide nucleic acids or proteins containing aldehyde groups.

Desheng has been developing and producing reagents for in vitro diagnosis of blood testing since 2005. It has deep research on the new Trinder's reagent, biological buffer and chemiluminescence reagent of chromogen substrate. It has a professional R & D team and production equipment. At present, the company's production equipment, process and process are newly upgraded. Each product is tested and certified by professional quality personnel to ensure product quality. You can buy it at ease. Welcome to inquire.