Steps of detecting the titer of heparin sodium with sheep plasma CAS NO.9041-08-1

1. Direct selling by manufacturers, preferential price and spot supply;

2. Desheng is a manufacturer specializing in the production of a full set of vascular additives. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been engaged in blood vessel collection industry for 14 years. Our products are independently developed and produced. We have the ability to solve most of the problems in vascular collection and provide customers with safer services.

4. Desheng began to engage in foreign trade in 2010. We have our own export rights.

Heparin is a substance with anticoagulant activity. The greater the content of heparin sodium in the solution, the stronger the anticoagulant effect. The titer of heparin sodium products produced in China is mostly determined by sheep plasma method. Its principle is to add the standard and test sample into the sheep plasma, and observe the coagulation degree of the standard and test sample heparin sodium added into the human tube after waiting for a certain time for the re calcification of the sheep plasma. If the standard and test sample are mixed into the same concentration and get the same coagulation degree, it means that their potency is also the same, so as to compare and determine the potency of unknown samples. The following are the steps of detecting heparin titer in sheep plasma.

1. Weigh the heparin powder to be tested according to the estimated titer;

2. Add 0.9% normal saline to dissolve heparin sodium powder and fix the volume to 100ml;

3. Take out 2ml of heparin solution after constant volume, and fix the volume to 50ml to obtain the solution to be tested;

4. Take out two groups of test tubes with 10 tubes in each group. The standard heparin solution and the solution to be tested injected into the two groups are 110 μ l to 200 μ L respectively (to avoid mixing, the amount added can be marked on the test tube);

5. Add 20 μ l of 8% sodium citrate solution and 0.8 ml of 25% calcium chloride to each tube;

6. Add 1ml of thawed and filtered sheep plasma into each tube;

7. Cover the rubber plug of each test tube, turn it over for 3-4 times and mix evenly to wet the inner wall;

8. Put it vertically into the water at 38 ℃ for 1 hour, take it out, observe the solidification degree of each tube, record the volume of heparin solution corresponding to 1 / 2 of the freezing point of the standard sample and the sample to be tested, and read it.

Calculation formula of potency of samples to be tested:

F = estimated potency of sample to be tested x 1 / 2 freezing point microliter of standard product ÷ 1 / 2 freezing point microliter of sample

Desheng company specializes in the production of blood collection additive series products. It has been more than ten years since the establishment of the company. The products mainly include heparin sodium, heparin lithium, serum separation gel, EDTA 2K / 3k, coagulant, coagulant powder and other products. It has a professional team to manage the R & D and production. If you are interested in Desheng's products, you can enter its official website to contact customer service for details.