The advantages and disadvantages of Tris buffer configuration method and application CAS NO.77-86-1

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 Tris Chinese name is Tris(hydroxymethyl)aminomethane; Tromethamine; Tromethamine; 2-Amino-2-(Hydroxymethyl)-1,3-Propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, corrosive to copper and aluminum, and irritating chemicals.
Tris has high buffering capacity, high solubility in water, and is inert to many enzymatic reactions, making Tris a very satisfactory buffer for many biochemical purposes. It is generally used to stabilize the pH of the reaction system, and has a strong buffer capacity between pH 7.5-9.0.
                          
Advantages of Tris buffer:
① Because of the strong alkalinity of Tris base, only this buffer system can be used to prepare buffers with a wide pH range from acidic to alkaline;
② Little interference to biochemical process, no precipitation with calcium, magnesium ions and heavy metal ions.
 
Disadvantages of Tris buffer:
① The pH value of the buffer is greatly affected by the concentration of the solution. When the buffer is diluted ten times, the pH value changes by more than 0.1;
② The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer solution, △pKa/°C=-0.031, for example: the pH of the buffer solution at 4°C is 8.4, then the pH at 37°C is 7.4, so it must be Prepared at the temperature of use. Tris-HCl buffer prepared at room temperature cannot be used at 0°C to 4°C;
③ It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;
④ This buffer has a certain interference effect on some pH electrodes, so an electrode compatible with Tris solution should be used.
 
Tris buffer configuration method:
There are two ways to prepare Tris-HCl buffer: respectively prepare 0.05 mol/L Tris and 0.05 mol/L HCl solution, and then mix according to the volume listed in the common table. However, since the standard concentration of dilute hydrochloric acid is not easy to prepare, another method is commonly used: take the preparation of 1 L 0.1 mol/L Tris-HCl buffer as an example: first weigh 12.11 g of Tris base and dissolve it in 950 mL to 970 mL of deionized water. , 4 N HCl was added dropwise while stirring, the pH value of the solution was measured with a pH meter to the desired pH value, and then water was added to make up to 1 L.
 
Tris buffer pH range:
Tris buffer is a widely used buffer in biochemical research, and its commonly used effective pH range is in the "neutral" range, such as:
Tris-HCl buffer: pH=7.5～8.5
Tris-phosphate buffer: pH=5.0～9.0
 
Application of Tris buffer solution:
It forms a buffer system with glycine to stabilize pH value in electrophoresis buffer; uses Tris-HCl buffer system to stabilize pH value in gels; it is widely used as a solvent for nucleic acids and proteins; the low ionic strength of Tris buffer can also be used in nematodes the formation of intermediate fibers; adding EDTA to Tris hydrochloric acid buffer to make "TE buffer", which can be used for DNA stabilization and storage; changing the pH-adjusted acid solution to acetic acid to obtain "TAE buffer", which can be replaced with Boric acid is available in TBE buffer. These two buffers are commonly used in nucleic acid electrophoresis experiments.

Desheng biotechnology has been established for decades, the research and development production of biological buffer has 15 years of experience, and Tris is the first research and development products of the company, as the company's core products, currently has more than one hundred countries in the world, reliable product quality, price concessions to talk about, are interested can inquire, DE sheng biotechnology welcome your calls.