Preparation of Tris base buffer CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Tris base buffer is usually referred to as the basic buffer of CAS no.77-86-1, which is prepared by dissolving Tris in water and adjusting pH with HCl. There are also Tris base salt, also known as TBS buffer, which is based on TB buffer and added with some inorganic salts.

Tromethamine Tris has better performance than phosphate buffer. The pH range of the buffer is 7-9, and the ionic strength is low. Unlike phosphate, it is easy to precipitate with Ca and Mg plasma. The pH range is suitable for most biochemical reactions. Therefore, Tris buffer is widely used in protein electrophoresis, Western blot and nucleic acid agarose gel electrophoresis.

Tris base buffer material production

Moreover, the price and cost of Tris is much lower than that of HEPES and pipes, so Tris buffer is an ideal choice for biochemical experiments. The dissociation constant pKa of Tris in solution is 8.1 (25 ℃), and the effective buffer range is 7.1-9.1, which is suitable for most biochemical experiments.

Tris aqueous solution itself is alkaline, and hydrochloric acid is usually used to adjust the pH value of buffer system

1. First, Tris was prepared into a certain concentration of aqueous solution, and then hydrochloric acid was dripped to adjust the pH value according to the experimental requirements to obtain Tris HCl buffer solutions with different pH.

2. By dissolving Tris HCl reagent powder in water directly, the buffer solution with corresponding pH value can be obtained without adjusting pH value.

Tris aqueous solution is alkaline and easy to absorb acid gas carbon dioxide in the air. When preparing the solution, pay attention to it in the fume hood, and do a good job in sealing operation when placing it.

Try to use hydrochloric acid instead of caustic soda to adjust pH. after overshoot and adjusting pH with NaOH or Tris HCl and adjusting pH with NaOH, the ionic strength of strong electrolyte will change. Depending on the intended use of Tris buffer, this change may have an impact.

When accurately adjusting the pH value of Tris buffer, Tris compatible electrodes, such as double junction or calomel reference electrode, should be used. Silver will gradually precipitate and block the electrode.

Whether Tris base or TBS, Tris HCl buffer, or Te, TAE, tbe buffer used in nucleic acid electrophoresis, the core raw material is Tris. Desheng's reagent raw material has advantages in quality stability and cost due to its centralized mass production.