How to Choose Commonly Used Biological Buffers CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 16 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Many biological experiments require the use of biological buffers to maintain an effective pH, which is important because proteins and enzymes are sensitive to changes in pH, so choosing the correct biological buffer for current and downstream experiments is critical. In this article, some important factors to consider when choosing a biological buffer will be briefly discussed, followed by a discussion on how to prepare buffers commonly used in life sciences. The pH buffer range data for biological buffers such as Tris, HEPES, and PIPES are shown in the table below.

biological buffer
pH buffer range
Tris
7.0-9.2
HEPES
6.8-8.2
PIPES
6.1-7.5
Tris/Tris-HCL
7.0-9.0

When choosing buffers, it is important to consider the advantages and disadvantages of each buffer. First, the pH range of the reagents you plan to use in your experiment must match the pH range of the buffer you choose. You can determine if they coincide by looking at the pH range of the buffer or by actually comparing the pKa of the buffer to the pH at which you want to hold the experiment. The pKa value should be within one unit of the desired pH.
Additionally, you must consider concentration, biological buffer toxicity, temperature and reactivity. The concentration of the biological buffer must be sufficient to account for the amount of acid or base you plan to use in your experiment. The more pH-altering ingredients you plan to use, the higher the biological buffer concentration required. Biological buffer toxicity is important because some buffers may be toxic to the cells being used, so if you are uncertain about toxicity, you can test the cells before using a biological buffer in your experiment. You should also make sure that the biological buffer you decide to use will not produce unwanted reactions in your experiment.

Today, there are more and more types of biological buffers and more and more fields of application. As a result, the biological buffers on the market have been in short supply for a long time, especially Tris buffers have been in a situation of price and no market for a long time. A company is recommended here.

 Hubei Xindesheng Materials Co., Ltd. specializes in the production of buffer products such as Tris, Hepes, and Pipes. Up to now, the company has been established for decades and has rich experience in the research and development and production of biological buffers. We can provide a large number of Tris and other types of biological buffers, and our company can provide product customization and professional after-sales service for customers, and can provide professional product sample testing services and packaging customization services for special customers. In any case, Desheng Company must be the best choice for you to buy biological buffers.