How does Tris maintain pH? CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Tris is called trimethylol aminomethane in Chinese; Aminobutanetriol; Bradykinine; 2-amino-2 - (hydroxymethyl) - 1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, irritating chemicals.

Tris crystal

Trimethylol aminomethane was used to prepare buffer solution in biochemistry and molecular biology experiments; Pharmaceutical intermediates; Used for the preparation of surfactant and vulcanization accelerator; Water soluble polymers containing Tris structural units were prepared as dispersants; Preparation of indoor formaldehyde adsorption materials, etc.

Tris is often used as biological buffer, and its pH value is 6.8, 7.4, 8.0, 8.8. Its structure formula, pH value changes greatly with temperature. Generally speaking, the pH value decreases by 0.03 when the temperature increases by one degree. Tris is widely used in the preparation of buffer solution in biochemistry and molecular biology experiments. For example, both TAE and tbe buffers (used for nucleic acid dissolution) commonly used in biochemical experiments need Tris. Because it contains amino groups, it can react with aldehydes. Tris is a weak base and its PKA is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH value of Tris base aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain the buffer solution with this pH value. At the same time, we should pay attention to the effect of temperature on pKa of Tris. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in such a solution to improve its solubility. People often add EDTA into Tris hydrochloric acid buffer to make "Te buffer", which is used for DNA stabilization and storage. If the acid solution of adjusting pH value is replaced by acetic acid, the "Tae buffer" (Tris / acetate / EDTA) is obtained, and the "tbe buffer" (Tris / borate / EDTA) is obtained by replacing it with boric acid. These two buffers are usually used in nucleic acid electrophoresis experiments. TAE and tbe made of Tris are the most commonly used reagents for DNA electrophoresis. Te (ph8.0) is mainly used for dissolving DNA（ Te is Tris and EDTA 1mtris-hcl6.8 and 1.5mtris-hcl8.8 are the most commonly used reagents for SDS-PAGE.

Tris should be stored in a cool, dry and moisture-proof warehouse at room temperature.

The purity of trimethylol aminomethane developed and produced by Desheng is more than 99%, with good water solubility and stable process. Package specification: large package 25kg plastic barrel, small package 500ml bottle.