Function, advantages and disadvantages of Tris derivatization buffer CAS NO.77-86-1

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Buffer solution is usually a solution composed of "weak acid and its conjugated base" or "weak base and its conjugated acid" buffer pairs, which can slow down the change of pH when adding a certain amount of other substances. The function of buffer is to adjust the pH value of the solution within a limited range so that the acidity of the solution to be tested meets the range specified by the analysis method. In biochemical experiments and related research work, a large number of buffers are often needed to maintain the pH of the experimental system. Tris and its derivative buffer are widely used in organic synthesis, coatings, fireproof materials, biochemistry and medicine. What are the advantages and disadvantages in practical application

Firstly, understand Tris buffer Tris, also known as trimethylolaminomethane. Tris buffer is more and more widely used, especially in nucleic acid DNA related research experiments, it has more advantages than PBS phosphate buffer. On the other hand, Tris is also an important raw material for the synthesis of another biological buffer taps. Tris has obvious advantages. Its pH buffer range is 7-9 and its alkalinity is strong. This kind of buffer can be used to configure pH buffer from acid to alkali; At the same time, because it does not precipitate with heavy metals, it can act together and has little interference with biochemical processes. Therefore, Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important applications. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also a protein electrophoresis buffer

It forms a buffer system with glycine in the electrophoresis buffer to stabilize the stability in the electrophoresis process

PH。

Tris buffer also has some disadvantages to be noted: 1. The pH of Tris buffer is greatly affected by the concentration; 2、

It is greatly affected by temperature effect. For example, Tris buffer with pH of 7.8 is at 4 ℃ at room temperature of about 25 ℃

It is 8.4 at 37 ℃ and 7.4 at 37 ℃. Therefore, when the buffer prepared at 4 ℃ is measured at 37 ℃, its hydrogen dissociation

The sub concentration increased 10 times. And it is at pH 7 Below 5, the buffer capacity is very poor.

Several common Tris derived buffers

1. TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservative, etc., pH7 4. Yes

Isotonic buffer salt solution commonly used in physics is mainly used for immunohistochemistry, in situ hybridization and enzyme-linked immunosorbent assay

During the test, the nonspecific binding antibodies in the immunoblotting experiment were cleaned.

2. TBS is Tris HCl buffer salt solution, which is isotonic salt solution plus Tris HCl buffer, mainly composed of

Tris HCl, NaCl and Tween20, pH 7 2-7.5, mainly used for immunohistochemistry and in situ hybridization,

In enzyme-linked immunosorbent assay and other experiments, the immunoblot was cleaned.

3. Te buffer is prepared by Tris and EDTA, an electrophoretic buffer, pH8 0, main

It is used to dissolve nucleic acids and can stably store DNA and RNA.

4. Tae buffer is a buffer composed of Tris, acetic acid and EDTA, pH7 2-8.4, it's a blockbuster

Buffer system widely used in short-time electrophoresis of segment DNA;

5. Tbe buffer is a buffer solution composed of Tris base, boric acid and EDTA (ethylenediamine tetraacetic acid)

Liquid. TBE buffer is usually used in agarose gel electrophoresis to analyze the amplification by PCR and DNA purification.

DNA products produced by case or DNA cloning.

Hubei xindesheng is specialized in biological buffer, blood collection additives, chemiluminescence reagents and luminescence

A high-tech company integrating R & D, production and sales of substrates and other products. After 15 years of rapid development, Desheng has a huge R & D team and experimental base. Now biological buffer products not only have a high share in the domestic market, but also have been sold to dozens of countries around the world. Desheng has established long-term and stable cooperation with many large biomedical technology enterprises.