Advantages and disadvantages of Tris and its derivatization buffer CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 16 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Buffer solution is usually a solution composed of "weak acid and its conjugated base" or "weak base and its conjugated acid" buffer pairs, which can slow down the change of pH when adding a certain amount of other substances. Buffer

The function is to adjust the pH value of the solution within a limited range to make the acidity of the solution to be tested meet the requirements of the analysis method

Scope of. In biochemical experiments and related research work, a large number of buffers are often needed to maintain the pH of the experimental system. Tris and its derivative buffer are widely used in organic synthesis, coatings, fireproof materials, biochemistry and medicine. What are the advantages and disadvantages in practical application

First understand Tris buffer

Tris, also known as trimethylolaminomethane, Tris buffer is more and more widely used, especially nucleic acid DNA

In relevant research experiments, it has more advantages than PBS phosphate buffer. On the other hand, Tris is also synthetic

Another important raw material of biological buffer taps.

Tris has obvious advantages. Its pH buffer range is 7-9 and its alkalinity is strong. This kind of buffer can be used only

The pH buffer in the range from acid to alkali is configured for the flushing solution; At the same time, because it does not precipitate with heavy metals, it can be one

It works and has little interference with biochemical processes. Therefore, Tris buffer is not only widely used as nucleic acid and egg

White matter solvent, there are many important uses. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of lamin in C. elegans. Tris is also one of the main components of protein electrophoresis buffer. It forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH in the electrophoresis process.

Tris buffer also has some disadvantages to be noted: 1. The pH of Tris buffer is greatly affected by the concentration; 2、

It is greatly affected by temperature effect. For example, Tris buffer with pH of 7.8 is at 4 ℃ at room temperature of about 25 ℃

It is 8.4 at 37 ℃ and 7.4 at 37 ℃. Therefore, when the buffer prepared at 4 ℃ is measured at 37 ℃, its hydrogen dissociation

The sub concentration increased 10 times. And it is at pH 7 Below 5, the buffer capacity is very poor.

Several common Tris derived buffers

1. TBS buffer is mainly composed of Tris, sodium chloride, BSA, preservative, etc., pH7 4. It is an isotonic buffer salt solution often used in biology. It is mainly used in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assay and other experiments to clean nonspecific bound antibodies in Western blot experiments.

2. TBS is Tris HCl buffer salt solution. It is isotonic salt solution plus Tris HCl buffer. It is mainly composed of Tris HCl, NaCl and tweet20, with pH 7 2-7.5. It is mainly used to clean the immunoblot in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assay and other experiments.

3. Te buffer is prepared by Tris and EDTA, an electrophoretic buffer, pH8 0, which is mainly used to dissolve nucleic acids and can stably store DNA and RNA.

4. Tae buffer is a buffer composed of Tris, acetic acid and EDTA, pH7 2-8.4, a buffer system widely used in short-time electrophoresis of large DNA fragments;

5. Tbe buffer is a buffer solution composed of Tris base, boric acid and EDTA (ethylenediamine tetraacetic acid). TBE buffer is usually used in agarose gel electrophoresis to analyze DNA products produced by PCR amplification, DNA purification protocol or DNA clone.

Hubei xindesheng is a high-tech company specializing in the R & D, production and sales of biological buffer, blood collection additives, chemiluminescence reagents and luminescent substrates. After 15 years of rapid development, Desheng has owned Pang

With large R & D teams and experimental bases, biological buffers have been formed, including Tris, HEPES, taps

Mops, caps, bicine, EPPs, pipes and other biological buffers. At present, biological buffer products not only have a high share in the domestic market, but also have been sold to dozens of countries around the world, and have established long-term and stable cooperation with many large biomedical technology enterprises.