The application of CHAPS buffer CAS NO.75621-03-3

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 17 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

CHAPS, the CAS number is 75621-03-3, its micelle molecular weight is small and the critical micelle concentration is 6mm, which can be completely removed by dialysis in the experiment. As a buffering agent, CHAPS can play a good buffering effect in the pH range of 2 to 12, and can maintain for a long time, so it is often used in pharmaceutical intermediates, laboratory chemicals and research reagents.
As a hydrophobic substance, the chemical properties of CHAPS are very stable, the ambient temperature has little effect on it, and it hardly reacts with metals. CHAPS does not contain persistent, bioaccumulative or toxic components, and is often used as a non-toxic stabilizer to regulate a class of organics necessary for the normal growth and metabolism of microorganisms. CHAPS buffer can more sensitively destroy the nucleus, dissolve and encapsulate the isolated and purified inner membrane proteins and receptors, and affect the interaction between proteins without changing the activity and function of the protein. CHAPS can be used to prepare vascular scaffold materials, remove cellular components, and obtain autologous acellular matrix.
CHAPS lysis buffer can avoid protein degradation and improve protein extraction efficiency, so it is often used to prepare thiocyanate samples for mammalian cell and tissue analysis. CHAPS can also inhibit apo-IDO1, promote protein iodination, divide and recover viral particles, and quick-freeze primary glioblastoma tissue samples.
Sometimes CHAPS is used in combination with other substances to achieve a better buffering effect, but the price is more expensive than a single buffer. For example, when analyzing caspase, CHAPS + HEPES combination buffer is often used; when detecting lipoprotein-related phospholipase A2, CHAPS and sodium n-nonane sulfonate are formulated into a solution to inhibit the esterase-like activity in serum; Enzyme nuclease incubation to degrade remaining DNA contamination etc.
As a professional source manufacturer of CHAPS, Desheng's scientific researchers continuously improve the production process, optimize the production process, and ensure the stability of the CHAPS white crystal powder produced. For more information about CHAPS, welcome to consult and purchase