What you should know about HEPES CAS NO.7365-45-9

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

HEPES, the Chinese name 4-hydroxyethylpiperazine ethanesulfonic acid, is a zwitterionic buffer. This buffer is often used in biochemical experiments to adjust the pH of the reaction system. For example: adding HEPES to virus preservation solution or cell preservation solution is non-toxic to cells and can maintain a constant pH range of the solution for a long time.

HEPES powder is resistant to high temperatures and has a melting point of 200°C. However, HEPES aqueous solution will produce toxic hydrogen peroxide under light conditions. Therefore, the use and storage of HEPES aqueous solution should be protected from sunlight.

HEPES has a wide range of uses, mainly in the following areas:
1. For cell culture: The decomposition ability of HEPES is less affected by temperature changes, which allows HEPES to maintain the structure and function of the enzyme at low temperatures.
2. For metal ion research: HEPES buffer is often used in the research of various metal ions. Based on the characteristics of the buffer, HEPES can be a good substitute for Tris and phosphate buffers in metal ion research.
3. Used in skin care products: HEPES is harmless to the human body and is safe and stable. Its pH-maintaining properties can help skin care products maintain the activity of microbial fermented extracts and prolong the effect; HEPES can promote the absorption of various components in skin care products and improve the skin Absorption rate of cosmetics; HEPES also has the effects of softening keratin, enhancing cell metabolism, and synergistic sun protection.
4. Used for virus preservation solution: HEPES can be used for non-inactivated virus preservation solution. The maintenance of pH of the solution by HEPES can increase the survival time of virus host cells in vitro, and maintain the integrity of virus nucleic acid and protein to the greatest extent.
5. Used for the preparation of gold nanoparticles: using HEPES to reduce excess metals at a specific pH value, the HEPES-NaOH reduction method is used to prepare gold nanoparticles. This method is simple to operate and has a rapid response.
6. For cell preservation solution: Cell culture solution generally relies on sodium bicarbonate and CO2 for buffering, but CO2 is unstable in the solution and easily dissipates, leading to an increase in pH. In this case, adding HEPES can improve the aforementioned problems and maintain a stable pH of the culture medium.

Our company can produce HEPES buffer products with excellent quality and reasonable price. For more information, please contact us: Hubei Xindesheng Material Technology Co., Ltd. terryge@whdschem.com.