What are the characteristics of HEPES buffer do you know CAS NO.7365-45-9

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2. Desheng is a manufacturer of biological buffer series products. Currently, there are many types of buffers produced, which can provide you with more comprehensive services;

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HEPES is an organic chemical buffer for amphoteric ions, belonging to one of the twenty Good buffers. It does not bind to metal ions and is highly soluble in water. It is often added to cell culture medium for use. Although it is not nutritious for cells, the addition of 10 -- 25 mm HEPES provides additional buffering capacity when cell cultures require prolonged operations outside of CO2 incubators to provide a favorable PH environment for cells.

HEPES buffer in barrels

The HEPES buffer has the following characteristics:

1. The pKa of HEPES depends on temperature: e.g. ① at 25ºC: pKa= 7.48 ② at 37ºC: pKa= 7.31

2, has good solubility in aqueous solution, aqueous solution colorless transparent;

3, stable chemical properties, no toxicity to organisms and no obvious infiltration of biofilm:

4, there is no characteristic absorption of ultraviolet wave, only weak absorption when the wavelength is less than 230-240nm, so it does not interfere with the usual photometric measurement.

5, is not susceptible to temperature, ion composition, concentration and other factors affecting the degree of dissociation

In addition to its use in cell media, HEPES is also very important in other biological studies because it does not bind to metal ions, so it can be used in many metal ion studies, and it is a good substitute for trimethylaminomethane and phosphate. HEPES is also commonly used as a buffer for protein deposition techniques in electron microscopy because it does not affect the metal substrate. HEPES has been evaluated and proved suitable for use with amphoteric compounds to produce a pH gradient less than 1 pH unit wide for isoelectric focusing applications. HEPES is also the preferred buffer among the recommended buffers for glutamate binding tests because it prevents binding to non-receptor materials.

Desheng is a professional manufacturer of biological buffer, which has been established for more than 10 years. With rich experience in r&d and production and product knowledge, desheng can provide a lot of technical support and after-sales guarantee for customers. Adhering to the business philosophy of taking morality as the foundation and sincerity as the first, Desheng has always put customers first. At present, Desheng produces a series of biological buffer products including HEPES, MOPS, TRIS, TAPS, CAPS, BICINE, EPPS, PEP and so on. Please feel free to contact us if you have any need!