Frequently Asked Questions about the Biological Buffer HEPES CAS NO.7365-45-9

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products, providing you with more comprehensive services with a variety of products;

3. Desheng has been in this industry for 16 years. Our product knowledge and production experience are very rich

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1. What is HEPES buffer
HEPES buffer is a biological buffer commonly used in cell culture and cosmetic additives. In chemistry, a buffer is a solution containing an acid and a base or a salt. The main purpose is to keep the hydrogen ion concentration at a level ideal for a particular purpose.
2. What is the main application of HEPES buffer
HEPES can maintain the ionic concentration of the solution at the level for the specific application. pH levels are maintained in this way. Very useful in cell culture, microbiology and histology, this buffer is classified as a zwitterionic sulfonic acid buffer.
3. What are the characteristics of HEPES buffer
HEPES has a molar mass of 238.3012 g/mol and is soluble in water. The buffer range of HEPES has obvious advantages in maintaining the physiological pH of cells, but HEPES buffer is very sensitive to light, and exposure to light produces hydrogen peroxide, which is toxic to cells due to its oxidative properties. So store and use this buffer in the dark whenever possible.
4. What is the pH range of HEPES buffer
The pH range of HEPES is 2.5 to 3.5 for acidic solutions and 6.8 to 8.2 for alkaline solutions. HEPES has two pKa constants, and their dissociation constants are called ions: pKa1 (25°C) = 3 pKa2 (25°C) = 7.5.
5. What is the role of HEPES buffer in cell culture
Growing cells in a laboratory setting has a variety of uses. It can be used for diagnosing certain types of diseases, genetic analysis, identifying microbial species, testing the efficacy of antibiotics, growing replacement skin tissue for skin grafts, conducting experiments on cancer cells, and extracting biomolecules. Therefore, maintaining the growth environment of cell culture is crucial for research analysis, and HEPES can maintain the pH value of the medium. To maintain the normal operation of the medium, the water in the medium, such as agar, and carbon dioxide generate bicarbonate, which has achieved the purpose of supplying nutrients.