What can the chemiluminescent substrate luminol detect? CAS NO.521-31-3

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Chemiluminescence is the light radiation generated by the absorption of chemical energy by a substance molecule. For a chemical reaction to become a luminescent reaction, two conditions must be met: first, the reaction must provide sufficient energy, and second, the chemical energy must be able to be used by a certain chemical. The molecular absorption of the species produces an electronic excited state with sufficient fluorescence quantum yield. So far, most of the chemiluminescence reactions studied are redox reactions, and most of them are liquid-phase chemiluminescence reactions. Luminol is one of the chemiluminescent reagents, and the luminous efficiency can reach 0.01. The luminescence mechanism of luminol and its derivatives is summarized as follows.
What are the derivatives of Luminol
Derivatives of luminol mainly include isoluminol, 4-aminohexyl-N-ethylisoluno, AHEI and ABEI, etc. Luminol can be oxidized by some oxidants under alkaline conditions, and a chemiluminescence reaction occurs, which emits chemiluminescence with a maximum emission wavelength of 425 nm. Using the inhibitory effect of organic compounds on the chemiluminescence reaction of luminol, it is of great significance to determine the organic compounds that have a quenching effect on the chemiluminescence reaction.

Luminol can detect and analyze the concentration of metal ions
The chemiluminescence reaction of luminol with hydrogen peroxide is slow under normal circumstances, but very rapid in the presence of certain catalysts. The most commonly used catalysts are metal ions. In a large concentration range, the concentration of metal ions is proportional to the luminescence intensity, so that the chemiluminescence analysis of certain metal ions can be carried out. This reaction can be used to analyze those organic compounds containing metal ions. very high sensitivity.
Luminol can be labeled to carboxylic acids and ammonia compounds for detection
Derivatives of luminol such as isoluminol (ABEI) are labeled on carboxylic acids and ammonia compounds, separated by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then subjected to alkaline conditions. Chemiluminescence detection by reaction with hydrogen peroxide-potassium ferricyanide. Other separation methods can also be used, such as labeling the newly synthesized chemiluminescent reagent isoluminol isothiocyanate to yeast RNA, separating by centrifugation and dialysis, and then performing chemiluminescent detection. In addition, N 2 (B2 carboxypropionyl) isoluminol was applied and its properties were studied.