MES buffers for cell culture media and chromatography CAS NO.4432-31-9

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 17 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

(N-Morpholinyl)ethanesulfonic acid MES is an excellent buffer, with a pH of 5.5-6.7, which is the high buffering force range of MES. Often used for chromatography and pH of buffer systems in cell culture media.
When (N-morpholino)ethanesulfonic acid MES isolates and purifies proteins in the laboratory, slight changes in pH can affect ionizable compounds, thereby affecting the separation and purification results. Therefore, it is necessary to add buffer to the experimental solvent to stabilize its pH. Because MES has low ionic mobility, low electrical conductivity and weak metal ion binding, it is often used in size exclusion chromatography, capillary liquid chromatography, hydroxyapatite chromatography, phosphocellulose column chromatography and hydrophobic chromatography. Usually, after the living cells pass through the metabolism, the pH value of the medium will shift to acidity, which will lead to the pH disorder of the whole system. Most cell culture media contain metal ions, and MES does not react with other ions except iron ions, so MES buffers are often added to the culture medium to stabilize the cell environment and make it in the best state of cell growth. Some fibroblasts have simple requirements on the medium and generally do not need to add additional buffers, but halophilic bacteria and yeast cells have high requirements on the growth environment, and need to add buffers to help adjust their growth environment. It should be noted that the amount of MES buffer added should be determined according to the capacity of the system. Over a long period of time, high concentrations of MES have latent toxicity to cells. Desheng recommends that the content of MES required by mammalian cells is less than 20mM, and the concentration of MES in plant media such as pine and fir trees is less than 10mM.
Desheng is one of the few raw material manufacturers of biological buffers in China. It has developed as many as 42 kinds of biological buffers, including a variety of common buffers and their derivatives, with a purity of more than 99%. Our professional sales staff will provide matching products according to customer needs. Welcome to consult and purchase.