Main components of cell lysis buffer Tris-HCl and EDTA CAS NO.25102-12-9

1. Factory production, preferential price, and spot supply of conventional products;

2. Support customization of serum separator, such as special customization of specific gravity and viscosity;

3. Desheng is a professional manufacturer of a full set of vascular additives, with a wide range of products. Can provide you with more comprehensive services;

4. Desheng has been engaged in vascular collection industry for 14 years. Our products are independently developed and produced. We have the ability to solve most of the problems in blood vessel collection and provide customers with safer services.

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Lysis refers to a state of cell division or rupture. Lysis buffer is a solution that helps to open cells. It can be used when some experimental analysis needs to extract cell contents. For example, scientists must use lysis buffer to assist in extracting DNA or protein from cells for analysis. There are many kinds of cell lysis buffer, among which the more common one is Tris-HCl buffer.

Tris-HCl can maintain the best pH value of cell lysis buffer, so it is often used as the main component of lysis buffer. Lysis buffer helps to open cells and screen substances in cells, such as salts, detergents, chelators and inhibitors, as well as some alkaline chemicals. It can not only distinguish and screen, but also remove these substances.

In addition to the biological buffer, the lysate also includes a chelating agent and inhibitor, such as ethylenediamine tetraacetic acid (EDTA), because this chemical combines with two positively charged metal ions (such as magnesium and calcium), so that they can not be used in other reactions. Many DNases (proteins that chew DNA) and proteases (proteins that chop other proteins) require magnesium ions to function. Therefore, EDTA helps to reduce the activity level of proteases or DNases by depriving these key components.

Desheng is a manufacturer specializing in the production of biological buffers and EDTA. At present, the biological buffer products produced include Tris, Tris-HCl, HEPES, taps, mops, caps, bicine, EPPs, PEP and a series of biological buffers. It has been established for more than ten years, has rich R & D and production experience and product knowledge, and can provide customers with a lot of technical support and after-sales guarantee. If you need anything, please feel free to contact us!