Application of HEPBS buffer CAS NO.161308-36-7

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 17 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

N-(2-hydroxyethyl)piperazine-N'-(4-butanesulfonic acid), or HEPBS for short, is often formulated as a buffer for use in diagnostic tests.
Physical properties of HEPBS
HEPBS has a CAS number of 161308-36-7 and is a white crystalline powder at 25°C. Its pKa value is 8.3, and this acidity value is in line with the environment in which cells grow. The HEPBS produced by our company is of reagent grade, and its aqueous solution is slightly turbid, which needs to be prepared and used. In practical application, after titration with sodium hydroxide solution as buffer, its UV absorbance is less than 0.1 in 33%W/W sodium hydroxide solution.
Chemical properties of HEPBS
The pH is in the range of 7.6-9.0, and the buffering capacity of HEPBS buffer is strong. However, it is greatly affected by temperature, so when HEPBS is used as a buffer, the influence of biochemical reaction temperature is considered. HEPBS is a zwitterionic compound with both positive and negative charges in its molecular structure. Its chemical structure contains a piperazine ring, which is chemically inactive, does not affect the activity of enzymes, and does not chemically react with most metals. Precipitation will not change the activity coefficient of the biochemical system, but it can be oxidized by strong oxidants and reduced by strong reducing agents.
Application of HEPBS
HEPBS is a member of the piperazine series buffer family, which can coexist with cations in the cell culture medium, and can provide the acidity environment required for cell growth, all of which are commonly used in cell culture medium to stabilize the pH value of the cell growth environment. Because, like EPPS, it has a high pKa value and is suitable for use on the skin. HEPBS can act as a buffer in cosmetic raw materials to adjust the acidity and alkalinity of various cosmetic ingredients.
The HEPBS produced by Desheng is only used for scientific research reagents. The purity of the powder is above 99%. When measuring its moisture by Karl Fischer method, the loss on drying is less than or equal to 1%. All products can be customized in small batches, and samples can also be provided for testing. . Welcome new and old customers to consult and purchase.