Interaction between bicine and metal ions CAS NO.150-25-4

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2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

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In most cases, metal elements do not exist in the form of white ions in organisms, but often form metal complexes with biological macromolecular ligands such as proteins and nucleic acids. The basic structural unit of protein is amino acid, and N, N-bis (2-hydroxyethyl) glycine (bicine), which was first synthesized by kiplranov in 1926, can be regarded as an analogue of helium based acid (glycine). It can be seen from the structure of bicine that bicine has the properties of amino acid and aminoethanol at the same time. It has a substituted amino group, a shuttle group and two hydroxyl groups as coordination groups, which can form complexes with metal ions. The most basic metal ion binding site in bicine molecule is its amino acid part.

In 1966, good et al. Found that bicine has a pH range of 7.6-9.0, which is suitable for the study of hydrogen ion buffers in biological systems. Bicine is soluble in water, insoluble in acetone, DMF, DMSO, DMAC and other organic solvents; Aqueous solution (25% concentration) is colorless and transparent, has less salt effect and is not easy to pass through the biofilm, so bicine is often called "good's" buffer. However, these pH buffers may interact with metal ions through the formation of complexes in solution, which makes some research results only effective when the buffer is at a certain concentration. For example, when calculating the binding constants of protein and metal ions, if the interaction between metal ions and buffer is not considered, a wrong conclusion will be obtained. Therefore, it is unreasonable to assume that bicine has little or no interaction with metal ions when it is used as buffer. Many experimental facts have proved that bicine can form stable binary and ternary complexes with metal ions. The stability of these complexes in solution has been studied extensively.

Therefore, more and more attention has been paid to the interaction between bicine and metal ions. The fact that bicine can form binary and ternary complexes reminds us that we should be cautious when using bicine as buffer in the presence of metal ions and biological ligands. In addition, because the two hydroxyl groups of bicine are weak when coordinating with metal ions, it is easy to form mixed coordination complexes when there are other ligands with strong coordination ability. Moreover, the metabolic reaction in vivo involves the balance between a variety of metal ions and a variety of donor molecules. In vitro study of the coordination balance between transition metal ions and two or more ligands is conducive to accurately explain the coordination phenomenon in vivo. Therefore, the interaction between amino acid analogues bicine and transition metal complexes needs further study.

Bicine produced by Desheng has purity ≥ 99%, chloride ion content ≤ 0.1% (chloride ion content can also be customized according to demand), good water solubility and stable process, which can ensure the appearance of pure white crystal powder.