Buffer Tris in protein purificationMain components of cell lysis buffer Tris-HCl and EDTA CAS NO.1185-53-1

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Lysis refers to a state of cell division or rupture. Lysis buffer is a solution that helps to open cells. It can be used when some experimental analysis needs to extract cell contents. For example, scientists must use lysis buffer to assist in extracting DNA or protein from cells for analysis. There are many kinds of cell lysis buffer, among which the more common one is Tris-HCl buffer.

Tris-HCl can maintain the best pH value of cell lysis buffer, so it is often used as the main component of lysis buffer. Lysis buffer helps to open cells and screen substances in cells, such as salts, cleaners, chelators and inhibitors, as well as some alkaline chemicals. It can not only distinguish and screen, but also remove these substances.

In addition to the biological buffer, the lysate also includes a chelating agent and inhibitor, such as ethylenediamine tetraacetic acid (EDTA), because this chemical combines with two positively charged metal ions (such as magnesium and calcium), which can prevent them from being used in other reactions. Many DNases (proteins that chew DNA) and proteases (proteins that chop other proteins) require magnesium ions to function, so EDTA helps to reduce the activity level of proteases or DNases by depriving these key components.

Desheng is a manufacturer specializing in the production of biological buffers and EDTA. At present, the biological buffer products produced include Tris, Tris-HCl, HEPES, taps, mops, caps, bicine, EPPs, PEP and a series of biological buffers. At present, it has been established for more than ten years. It has rich R & D and production experience and product knowledge, and can provide customers with a lot of technical support and after-sales guarantee. If you need anything, please feel free to contact us!