MOPS (3-morpholine propane sulfonic acid) is a Good's buffer, a zwitterionic buffer discovered by Good et al. in the 1960s, with a pKa value of 7.14 at 25°C and an effective pH adjustment range of 6.5-7.9. MOPS does not form complexes with most metal ions, and is suitable for buffers in solution systems containing metal ions. MOPS is commonly used in the culture medium of bacteria, yeast and mammalian cells. MOPS is also used as a running buffer in electrophoresis and a buffer in protein purification chromatography.
Desheng MOPS small package and large package diagram
The main application areas of MOPS as a buffer are as follows:
1. In vitro diagnosis: biochemical kits, DNA/RNA extraction kits and PCR kits.
2. Protein/nucleic acid separation and purification: electrophoresis running buffer, chromatography buffer.
3. Cell culture medium: the buffer system of the culture medium.
4. Biosynthesis: the buffer used in biosynthesis.
Commonly used 10×MOPS Buffer configuration methods are as follows:
1. Weigh 41.8 g MOPS and dissolve it in about 700mL DEPC treated water;
2. Use 2 N NaOH to adjust the pH to 7.0;
3. Add 20mL of 1M NaOAC treated with DEPC and 20mL of 0.5M EDTA (pH8.0) to the solution;
4. Dilute the volume to 1 L, filter with a 0.45 μm filter membrane to remove impurities, and store at room temperature away from light.
Precautions for MOPS buffer:
1. MOPS biological buffer reagent is a zwitterionic buffer. If the dosage of MOPS is very small each time, it is generally used appropriately after appropriate dispensing.
2. Generally, the color of the biological buffer is very easy to change to yellow when it meets the light. The light yellow can be used completely, and it is not suitable to use after the color is too dark.
3. Attention should be paid during use. For safety and health, please wear laboratory clothes and disposable gloves for operation. If you accidentally contact your eyes, rinse with plenty of water and seek medical attention immediately.
Desheng is a diagnostic reagent raw material manufacturer with many years of experience in R&D and production. The MOPS products produced are of high purity ≥99%, good water solubility, stable production technology, and a strict quality management system to ensure that it provides customers with high-quality products and services. In addition to MOPS, TRIS, CAPS, TAPS and other buffer products, it also produces blood collection tube additives, chemiluminescence reagents, color substrates and other products. Welcome to inquire and order!