Application of 3-Morpholinopropanesulfonic acid （MOPS） CAS NO.1132-61-2

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Mops, namely 3-morpholine propanesulfonic acid, is a widely used and very important buffer. Mops itself is a good's buffer, with a buffer

range of 6.5 ~ 7.9. It has good pH stability and high polarity. It is inert to a variety of chemical reagents and enzymes, does not

participate in or interfere with the biochemical reaction process, and has no inhibitory effect on enzyme chemical reaction. Therefore, it

can be specially used in the research of organelles and highly variable, pH sensitive proteins and enzymes.

Main application fields of mops

1. Used as running buffer in electrophoresis and protein purification in chromatography;

2. A variety of agar media were prepared for the culture of bacteria, yeast and mammalian cells. However, higher than 20 mm

It is not recommended for mammalian cells;

3. It can be used as lysis buffer for E. coli cells;

4, as eluent in gel filtration chromatography.

5. It was used in Northern hybridization as a buffer for RNA isolation and membrane transfer;

6. It is suitable for the determination of biquinolinic acid (BCA);

7. It is used for electron transfer and phosphorylation of chloroplast thin layer preparation.

Precautions for use of mops

1. Mops is a kind of zwitterionic buffer. If the dosage of mops is very small each time, it is generally reasonable to use it after proper subpackage.

2. It is very easy for the biological buffer to turn yellow when it meets the light, and the light yellow one can be used completely.

If the color is too dark, it should not be used again.

3. Special attention should be paid to the use of mops biological buffer reagent. The production safety and personnel health must not be

careless, so the experimental clothes should be worn well, and disposable gloves should be worn to operate. Once the solution

splashes to the eyes, or slightly contacts, you must immediately use a lot of water to wash, and immediately to the hospital.

Production method of mops

The mainstream production method of mops is completed in the following six steps, which can not only save costs, reduce pollution, but

also avoid safety risks. This method of preparing mops uses water as solvent, and no organic solvent is used in the process. At the

same time, the production cost is reduced, the risk of harm to the health of operators is reduced, and there is no trouble of recycling

organic solvents, and there is no problem of environmental pollution. The main steps are dissolution of morpholine, reaction to produce

3-morpholine propanesulfonic acid aqueous solution, dehydration to obtain 3-morpholine propanesulfonic acid crude product,

recrystallization of crude product, crystal filtration and re concentration, cooling, crystallization to obtain 3-morpholine propanesulfonic acid

Desheng is a diagnostic reagent raw material manufacturer with many years of R & D and production experience. The purity of mops

products is more than 99%, with good water solubility and stable production process. It has a strict quality management system to

ensure that it can provide customers with high-quality products and services. At present, the products of the company have been sold to

many countries around the world. Welcome to call for details!