purpose of Tris buffer CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Trimethylaminomethane is a zwitterionic buffer, which is often used in biology and scientific research experiments. Of course, there are many applications in medicine and industry. So what are the specific uses of different Tris buffers, how to use Tris buffers, and understand the characteristics of Tris and different biotechnology, such as electrophoresis or chromatography.

Basic information of Tris:

CAS No.: 77-86-1

Molecular weight: 121.14 g / mol

Formula: C   four   H   eleven   NO   three

Available pH range: 7.2-9.0

pKa（25 ° C）：8.06

Maximum wavelength（ λ max) λ: 260 nm Amax: 0.10： λ: 280 nm Amax: 0.08

What is Tris recommended for

Commonly used in gel electrophoresis buffer (TAE or TBE).

Usually used for preparation of Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE)

Anion exchange chromatography

Used to evaluate bacterial endotoxin

For capillary electrochromatography (CEC), due to its low ion mobility

Used for metal complexation

It can be used to correct acidosis