Tris buffer was used in Western blot CAS NO.77-86-1

1. Factory production, more preferential prices, regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products. There are many kinds of products, which can provide you with more comprehensive services;

3. Desheng has been in the industry for 16 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Western blot experiments usually bring frustration in the laboratory. It is a hard work to obtain perfect results. When trying to visualize high molecular weight proteins, it will be more difficult. In the following, we will bring you several biological buffers that can be used in Western blot experiments.

The selection of biological buffer in Western blot experiment is very important. In the following, we will bring you the effects of three common biological buffers on Western blot, so as to select a more suitable one.

Tri's-glycine has alkaline pH (8.6) and short shelf life, and its pH will increase during electrophoresis (peak is 9.5), resulting in protein degradation and low resolution imprinting.

BIS Tris is more acidic (6.4), increasing stability and shelf life. It requires additional antioxidants in the buffer to maintain protein reduction, because common reductants do not move with proteins in the system.

The pH value of Tris acetate was kept at about 7, and macromolecular proteins with higher resolution were isolated than bis tri's or Tris glycine. Therefore, Tris acetate is recommended for Western blot experiments. Remember that after they use Tris acetate buffer, mops or MES buffer will not work.

In these experiments, the demand for Tris buffer is very large. After all, these solutions need to be prepared with tris buffer. Tris buffer is also widely used in other fields. If you are interested in the professional knowledge of Tris buffer, you can enter our official website for more information. Of course, if you want to buy Tris buffer or other biological buffer, you can also contact our company.

Our company is a manufacturer specializing in the production of biological buffer, with many years of product production and R & D experience, high production efficiency and good product quality. In addition to Tris, our company also produces HEPES, mops, caps, taps, pipes and other buffers, and our company can provide customers with product customization and professional after-sales service, as well as professional product sample testing service and packaging customization service for special customers. In any case, Hubei xindesheng Material Co., Ltd. must be your best choice to buy biological buffer.