Tris buffer derivates TEs and BIS Tris CAS NO.77-86-1

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Tris  is a commonly used biological buffer, which is widely used in the field of biochemistry. At the same time, it is also an important chemical raw material. For example, the common Tris HCl, TAE, TES, BIS Tris, etc. are synthesized from it. This paper will introduce TEs and BIS Tris derivatives in detail.

Tris powder

1. Tes (tris ethanesulfonic acid)

Chinese name of TEs: tris ethanesulfonic acid, CAS No.: 7365-44-8. The structure of TEs is similar to trizma. It is one of the series of ethanesulfonic acid physiological buffers developed by good and meets various standards of "good" buffer. Although the structure is neutral molecule, it is still zwitterionic in the solution. The pKa of TEs is 7.4 (physiological pH), so it is a widely used biological buffer reagent.

TES is suitable for various cell culture systems that need divalent metal ions, while many other buffers (such as citrate or phosphate) will produce chelation or precipitation reaction, which makes it unable to meet this requirement. In addition, TES is very helpful to study the oxidation reaction of succinic acid.

2. BIS tris (BIS trimethyl hydrochloride)

BIS Tris Chinese Name: bis (2-hydroxyhexyl) amino (trimethylol) methane, CAS No.: 6976-37-0, is an inert zwitterionic buffer, and its dissociation constant changes only slightly with the change of temperature. BIS Tris can slightly ionize hydrogen ions, which is weakly acidic and suitable for weakly acidic buffer system. Due to the introduction of two hydroxyethyl groups in Tris, there are many atoms providing lone pair electrons, and it has the ability to complex some metal ions, which is similar to EDTA or bicine.

BIS Tris is suitable for the separation of hemoglobin. It can protect the stability of hemoglobin during freeze-drying preservation. It can be used as an effective buffer for muscle enzyme activity analysis. 2-D gel electrophoresis is used as cathode fluid in isoelectric focusing electrophoresis (IEF).

Hubei xindesheng Material Technology Co., Ltd. is a manufacturer of diagnostic reagent raw materials. It can provide a variety of biological buffers, including Tris, Tris HCl, BIS Tris, bicine, taps and other reagents. If you need to buy, you are welcome to contact us at any time!