Importance of Tris and EDTA (TE) buffer in DNA extraction CAS NO.77-86-1

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Tris and EDTA are the two main components used in the whole DNA extraction experiment. Tris and EDTA are suitable for the preparation of lysis buffer, elution buffer and washing buffer. Tris-edta buffer is abbreviated as te buffer. Its main function in DNA extraction is to dissolve DNA into liquid form. It is also used as lysis buffer.

Tris buffer

1. Tris buffer

Tris is a (hydroxymethyl) aminomethane with molecular formula (hoch2) 3cnh2. It is a white crystalline substance in appearance. The main role of Tris in biological practice is to maintain the pH value of the solution. It is mainly used in biochemistry, molecular biology and chemical experiments. The pKa value of Tris is 8.07 at 25 ℃. It can also increase the permeability of cell wall. It is the main component of DNA extraction buffer and is conducive to the cleavage of cell wall and nuclear membrane.

2. Ethylenediamine tetraacetic acid (EDTA)

EDTA, ethylenediamine tetraacetic acid (EDTA) is widely used in medicine, industry, laboratory and cosmetics. It is a white powdery substance and water-soluble organic molecule. However, it can be dissolved in water with high pH (pH close to 8.0). The chemical formula of EDTA is c10h16n2o8. It acts as a chelating agent in DNA extraction. It can chelate the existing metal ions into the enzyme. Metal ions are cofactors that increase enzyme activity. By chelating metal ions, it inactivates the enzyme and reduces the activities of DNase and RNase.

Te buffer, as the component of extraction buffer, elution buffer and storage buffer, can cooperate well with protease K DNA extraction method in the study. Tris and EDTA products produced by Desheng Biochemical Institute have high purity and stable performance. It has been highly praised by customers in the market. Welcome to order if you need!