HEPES CAS7365-45-9 CAS NO.7365-45-9

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

HEPES application:
1. HEPES for metal ion research
It can be combined with metal ions, and most of the metal ion research uses hepes buffer for experiments;
2. HEPES for environmental analysis and biological research
As a binding buffer and eluent in cation exchange, and used for grinding in plant research and development. In addition, hepes is also an electrophoresis buffer, and is often used in electroporation production methods.
3. HEPES is used in skin care products
HEPES is safe to the human body, non-toxic, good in physiological compatibility, and makes the product anti-aging effect lasting for a long time, so it is often used as a stabilizer and active agent in cosmetics; as a penetration enhancer, it can promote the development of various functional components in cosmetics Transdermal absorption, compared with azone-based penetration enhancers, has the characteristics of short onset time, less dosage, and high penetration rate; it has the effect of softening cutin, promoting cell metabolism, interacting with other ingredients, and can gently remove old Old keratinocytes can effectively promote the renewal of basal cells, achieve smooth, soft skin, and brighten the skin; HEPES has the effect of synergistic sun protection and strengthening the skin barrier.
4. HEPES is used for non-inactivated virus preservation solution
It increases the in vitro survival time of virus host cells after sampling while maintaining the pH value, retains the integrity of viral nucleic acid and protein to the greatest extent, and improves detection accuracy.
5. HEPES is used in the preparation of gold nanoparticles
The HEPES-NaOH reduction method is used to prepare nano-gold particles, which has simple operation, fast reaction, easy control, few by-products, and is environmentally friendly.
6. HEPES is used for cell preservation solution
To improve the problem of too rapid pH change of the solution, and to maintain a relatively constant pH value of the culture solution, HEPES will increase the osmotic pressure of the cell culture system to a certain extent. When the concentration is too high, it may have certain cytotoxicity.

The characteristics of a good HEPES buffer:
1. The pKa value is between 6.0 and 8.0;
2. Has high solubility in water;
3. It has membrane impermeability and is not easy to penetrate biological membranes;
4. It has limited impact on biochemical reactions, is resistant to chemical and enzymatic hydrolysis, and does not form complexes or precipitates with metal ions;
5. Very low absorption of visible light and ultraviolet light;
6. Ion concentration, solution composition and temperature have little effect on dissociation;
Precautions:
1. HEPES powder is resistant to high temperature, the melting point is as high as 200℃, so it will not be degraded by autoclaving;
2. If the HEPES water solution is exposed to ambient light for three hours, cytotoxic hydrogen peroxide (H2O2) will be produced;
3. The HEPES water solution must be kept away from light so as not to affect the experimental results;
4. After being configured as a solvent, it should be stored at 4°C, and it is recommended to use it in a short period of time for better results;
5. When HEPES powder is placed in a dry room, it should not be exposed to direct sunlight for a long time.