Do you know the application of the biological buffer HEPES in cell culture? CAS NO.7365-45-9

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

The Chinese name of the biological buffering agent HEPES product is: N-hydroxyethylpiperazine-1-ethanesulfonic acid, and the appearance of the product is white crystalline powder. The biological buffering agent is made of some chemical products as raw materials, under high temperature and certain pressure, and Under the action of a catalyst, it is refined through a multi-step chemical unit. It is a high-quality, high-tech biochemical product in biological research. It is widely used in clinical diagnostic reagents, cell culture medium biological research, pharmaceuticals, cosmetics, and many other fields. Today we will mainly talk about the application of the biological buffer HEPES in cell culture.
      
In the cultivation of most cells, the required pH value of the cells is between 7.2-7.4. If HC03 or CO2 is used as the cell culture buffer, the pH value is very unstable, and it is easy to be alkaline after storage for a certain period of time. , So it will affect the survival rate of cells, even if they survive, its activity rate is also very low, you can add HEPES to the culture medium,

Hepes is a non-ionic amphoteric buffer, which has a good buffering capacity in the pH range of 7.2-7.4. The biggest advantage of HEPES is that it can maintain a relatively constant pH during open culture or cell observation, and can prevent pH oxidation in the medium Increase the pH to maintain the pH at about 7.0. In principle, HEPES can be used as a buffer to replace bicarbonate to relieve the restriction of the need for high-concentration CO2 culture environment, so it is deeply loved by cell culturers.

Hubei Xindesheng Materials specializes in the research and development and production of biological buffers. It has been established for decades. The biological buffer products currently produced include a series of biological buffers such as TRIS, HEPES, TAPS, MOPS, CAPS, BICINE, EPPS, PEP, etc. Available for customers to choose. The price is favorable, the supply is sufficient, please feel free to contact us if necessary!