Biological buffer HEPES VS PBS who is more widely used CAS NO.7365-45-9

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products, providing you with more comprehensive services with a variety of products;

3. Desheng has been in this industry for 16 years. Our product knowledge and production experience are very rich

4. Desheng has been engaged in foreign trade since 2010. We have our own right to export.

Biological buffer is a chemical substance that can stabilize the pH value in the required range. There are many types of it, and the pH value range is also different. Therefore, it is widely used in various experiments. After adding a buffer, the acid and alkali in the experiment can be changed. sex, which plays a crucial role in the success of the experiment. In fact, biological buffers have not been widely used before 1996. The traditional buffer pH value cannot effectively buffer higher than pH 7.5, so it is limited to many applications.
In 1996, Dr. Norman Good developed zwitterionic buffers, which can effectively solve some problems encountered in traditional buffers, because some of the biological buffers developed this time are relatively close to our physiological pH value, and It is non-toxic to cells and will not be absorbed through the cell membrane. Among them, HEPES and PBS are two commonly used buffers.
Comparison of HEPES buffer and PBS buffer:
1. Effective PH value range
HEPES: 6.8-8.2 PBS: 5.8-8.0
2. The effect on cells
HEPES: Non-toxic to cells, maximum cell density and viability is seen when using the HEPES buffer system.
PBS: Non-toxic to cells, dilution of cell culture samples in PBS may inadvertently reduce cell viability.
3. Relationship with metal ions
HEPES: Does not form complexes or precipitates with metal ions.
PBS: It can form complexes or precipitates with certain metal ions.
4. Changes under freezing conditions
HEPES: The pH of the solution will not change under freezing conditions.
PBS: Refrigeration of the solution can cause significant changes in pH.
Based on the above points, it is not difficult to see that HEPES buffer has certain advantages compared with PBS buffer. HEPES is also the best choice for cell culture buffers. Desheng Biochemical is a manufacturer specializing in the production of HEPES. Since its establishment in 2005, it has been Committed to the research of biological buffers, only to manufacture high-quality products, if necessary, please feel free to visit our official website for consultation! We look forward to hearing from you.