Application advantages of hydroxyethylpiperazine ethanesulfonic acid HEPES CAS NO.7365-45-9

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products, providing you with more comprehensive services with a variety of products;

3. Desheng has been in this industry for 16 years. Our product knowledge and production experience are very rich

4. Desheng has been engaged in foreign trade since 2010. We have our own right to export.

 Hydroxyethylpiperazine ethanesulfonic acid HEPES is a weak acid. It is used as a zwitterionic buffer in biochemical detection. Compared with other buffers, it has the advantage of no cytotoxicity, so it is widely used in various cells, viruses Although it is not as widely used as Tris, it has significant advantages in specific situations.
1. The advantages of HEPES in production:
The production process of HEPES is pollution-free and waste-free, which can realize clean production and has a good scientific and technological demonstration effect; and can be extracted, crystallized, coupled and separated with low-cost chemical raw materials under the action of high temperature, certain pressure and catalyst. Refined by multi-step chemical unit operation. It can be synthesized from hydroxyethylpiperazine and sodium chloroethanesulfonate, or from sodium isethionate and ethylpiperazine. Originally, its production technology used organic solvents (from solid state to liquid phase-wet process) for trial production. The wet process process uses a large amount of solvent, consumes high energy for evaporation, and the solvent is easy to remain. Product quality improved.
2. HEPES as a buffer for enzymes at low temperature
Usually, the use conditions of biological buffers are room temperature 25 degrees Celsius, or higher temperature (depending on the optimum pH range of the enzyme), but sometimes experiments at low temperature are required, and some buffers are obviously not suitable for use due to the influence of low temperature. The decomposition ability of HEPES can increase with the increase of temperature and weaken with the decrease of temperature, but compared with other buffers, its decomposition constant does not change much with temperature, which makes HEPES buffer a better solution at low temperature. Buffers that maintain the structure and function of enzymes.
3. HEPES has no toxic side effects on cells
HEPES buffer can control a constant pH range for a long time. Low concentration of HEPES has no toxic effect on cells. It is often used in cell culture. The pH environment required by bacteria and viruses.
In addition to being used in cell culture fluid and virus preservation solution, HEPES is also used in many in vitro diagnostic kits containing enzymes, and even cosmetics containing buffering agents. Desheng is a manufacturer of biological buffers, which can provide a variety of biological buffers such as HEPES in sufficient quantities.