Application of chelating pH buffer Bis-Tris to bind lead ions CAS NO.64431-96-5

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1,3-Bis(tris(hydroxymethyl)methylamino)propane, referred to as BIS-TRIS, is a derivative of TRIS buffer and a highly efficient chelating pH buffer. Bis-Tris can identify calcium binding sites in cellular proteins that are most vulnerable to lead competition.
Positive divalent lead ion is a xenobiotic metal ion, and the exposed lead ion has certain harm to the environment and human body. Exposure to young children can cause acute or chronic poisoning and even irreversible neurological damage. So finding protein-binding sites that are vulnerable to lead ion attack is crucial. In a non-chelating environment such as MES buffer, identification of these proteins is physiologically relevant
The lead ion binding site is challenging because lead ion interactions can be both specific and opportunistic and often exhibit higher affinity than natural metal ions. The chelating agent Bis-Tris can be used to probe high-affinity lead ion sites and determine their specific functional roles.
Bis-Tris has five hydroxyl groups and one tertiary amino group, all of which may act as ligands for various divalent metal ions. Bis-Tris forms weaker but stable complexes with lead ions compared to other strong chelators such as EDTA. In addition, Bis-Tris is a highly adaptable chelator because the number of functional groups involved in the coordination of metal ions may vary depending on the offered competing ligands.
presence varies, for example, by proteins, lipids or other small molecules. Using Bis-Tris is not only able to distinguish high-affinity and low-affinity lead ion sites. The use of the chelating pH buffer Bis-Tris in metal binding studies can provide valuable insights into the mechanism of metalloproteins. Bis-Tris not only does not affect the binding of lead ions to high-affinity sites, but also provides additional ligands for protein-bound metal ions. But non-chelating MES buffers have weak interactions with the binding site.
From a lead ion toxicity perspective, membrane interactions of lead ion complexes are unlikely to occur in a cellular environment rich in Bis-Tris chelators and metabolites. In addition to its use in metallic lead, Bis-Tris buffers have a wide range of other applications. If you need a professional manufacturer of Bis-Tris, look for Desheng, our company can provide you with a full range of services.