Chemiluminescence immunoreagent Luminol CAS NO.521-31-3

1. As a manufacturer rather than a trader, we can provide more reasonable and competitive prices, flexible inventory and strong manufacturing process control, so the batch difference is smaller and the after-sales service is stronger;

2. Desheng is a manufacturer of chemiluminescence immunoreagent series. We are able to provide you with one-stop purchasing experience;

3. Desheng has been in this field for 16 years. Our products are independently developed and manufactured, and we have accumulated a lot of clinical experience, which can provide strong problem-solving ability for our end users;

4. Desheng has been exporting since 2010. We have strong logistics capabilities, including packaging, selecting appropriate agents and freight forwarders, customs declaration and customs document preparation.

Compared with traditional techniques such as radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA) is a new technology based on chemiluminescence (CL) reaction, which has attracted much attention in recent ten years. Due to its wide dynamic range and high sensitivity, this technology has quickly become the main method of clinical immunological analysis. This method is widely used in the detection of tumors, infectious diseases, metabolic diseases and pregnancy.

There are three chemiluminescence immunoassay methods:

I Direct chemiluminescence immunoassay

Commonly used markers: isoluminol and acridine ester. These markers can produce special luminous groups and directly participate in the reaction in the analysis process.

1. Direct labeling chemiluminescence immunoassay with acridine ester: after immune reaction with the corresponding antigen (antibody) in the sample to be tested, a solid-phase coated antibody acridine ester labeled antibody complex is formed. At this time, only oxidant (H2O2) and NaOH are added to form an alkaline environment, and acridine ester decomposes and emits light without catalyst.  

2. Chemiluminescence immunoassay of luminol labeling: the luminescence of luminol is oxidation reaction luminescence. In alkaline solution, luminol can be oxidized and luminescent by many oxidants, among which H2O2 is the most commonly used. Due to the slow luminescence reaction, some enzymes or inorganic catalysts need to be added. The enzymes are mainly horseradish peroxidase (HRP), and the inorganic ones include O3, halogen, Fe3, Cu2, CO2 and their complexes.

II Indirect luminescence immunoassay

The commonly used markers are horseradish peroxidase (HRP, the commonly used substrate is luminol or its derivatives, such as isoluminol) and alkaline phosphatase (ALP, the commonly used substrate is 1,2-dioxane derivative, amppd). These markers participate in the luminescence reaction as a catalyst or energy transfer receptor of the luminescence reaction.

III Electrochemiluminescence immunoassay

The common marker is tripyridine ruthenium (the common substrate is tripropylamine).

The three luminescence technologies have their own advantages and are widely used in different disease diagnosis fields.

Chemiluminescence immunoassay is of great significance in the field of clinical diagnosis, and chemiluminescence reagents play a great role. The isoluminol and acridine ester series products produced by Desheng biochemical are of stable quality and supported by excellent scientific research and after-sales team. Welcome to inquire!