Characteristics and application of luminol reagent CAS NO.521-31-3

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Luminol  is a chemiluminescent reagent, also known as luminescent ammonia. Usually, the material state at temperature is yellow crystal or beige powder, and its properties are very stable. Generally speaking, luminol reagent is the mixture of luminol and hydrogen peroxide. Luminol solution has significant strong acidity, which will stimulate human organs, such as eyes, mouth and nose, skin, respiratory tract and so on

It has many application fields and is very useful. For example, in forensic medicine, luminol is often mixed with a mixture of sodium hydroxide and hydrogen peroxide, which can be used as a blood test in modern criminal investigation. The principle is actually very simple. This is because hemoglobin contains iron, which can catalyze the decomposition of hydrogen peroxide, convert hydrogen peroxide into liquid water and monooxygen, and then oxidize luminol to make it glow normally. Through this theory, it can be used to identify blood stains.

Even if the blood stains at the crime scene have been wiped or removed, the investigator can still find them with luminol. In fact, the investigator sprays luminol and excitation solution in the area to be investigated, and the iron in the blood immediately catalyzes the luminol luminescence reaction to produce blue light. The amount of catalyst required for this reaction is very small, so luminol can detect trace blood. The luminescence lasts for about 30 seconds, which can be observed through long exposure photos, and the surrounding environment can not be too bright.

The phenomenon of light radiation in the process of chemical reaction is called chemiluminescence. Chemiluminescence analysis method can determine the content of chemical reaction components. This trace and trace analysis method has the advantages of fast analysis speed, high sensitivity, wide linear range and simple instrument and equipment. The biggest feature is that the emitted photons can be measured without other light sources, so as to avoid the possibility of being affected by the light source background and greatly improve the signal-to-noise ratio. It analyzes the fields of medicine, food industry, biology, life substances and no organic, inorganic and transition metal elements in environmental monitoring.

Luminol is the most commonly used chemiluminescent reagent because it has the characteristics of being oxidized by other substances to produce luminescence. At the same time, it has many advantages, such as high luminescence quantum yield, easy synthesis, good water solubility and so on. It can combine with oxidant to form a variety of luminous systems. Most luminol chemiluminescence systems are often used in the determination of biochemical experiments. In addition, they can also be used as detectors for some separation technologies such as capillary electrophoresis and high performance liquid chromatography. If you need anything, you can contact Desheng.