Determination of free fatty acids by chromogen substrate tops CAS NO.40567-80-4

1. Direct selling by the manufacturer, independent R & D and production, quality assurance and high cost performance;

2. Purity ≥ 99%, good water solubility, stable process and high sensitivity. Creatinine is commonly used in renal function series.

3. Desheng is a manufacturer specializing in the production of chromogenic substrates (New Trinder reagent), with a wide range of products. We can provide you with one-stop procurement services;

4. As a manufacturer rather than a trader, we have the ability to provide more reasonable and competitive prices. We have flexible inventory, professional production team, strict quality inspection, high-quality after-sales service and no batch difference;

5. Desheng has 14 years of production and R & D experience in this field. All our products are independently developed and produced, and have accumulated rich clinical experience, which can provide our end users with strong problem-solving ability.

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The determination of free fatty acids (FFA) in serum is an important biochemical index. FFA is closely related to human health. Because the serum composition is complex, there are many kinds of FFA and there are many interference factors in the detection, the chromogen substrate tops is usually used to determine FFA.

Chromogen substrate TOPS reagent

Steps of FFA determination by chromogen tops:

1. First add the buffer weighed according to the formula proportion into the container, then add ATP, coenzyme A, 4-aminoantipyrine, ionic activator (magnesium chloride), stabilizer (BSA) and preservative successively, add an appropriate amount of water, then add surfactant, adjust the pH to ph6-9 with concentrated hydrochloric acid, finally add acyl coenzyme A synthase, stir and filter, Add distilled water to the obtained filtrate to obtain reagent a.

2. Add buffer into the container, then add chromogen tops, water and surfactant successively, adjust the pH to pH 6-9, finally add HRP and acetylcoa oxidase, then stir and filter, and then add distilled water to the filtrate to obtain reagent B quantitatively.

3. Fill the above reagents into vials and store them at 2-8 ° C. Mix reagent A with the sample in a certain proportion, incubate for a certain time, and read the absorbance value A1; Add reagent B, mix well, incubate for a certain time, and read the absorbance value A2. FFA concentration in sample = concentration of standard solution Χ( Λ Α_/ Λ Α_#)， Λ Α_=Α 2-A1。

Preparation buffer:

First add HEPES buffer (good's buffer, i.e. zwitterionic buffer, including HEPES, Tris, MES, mops, etc.) weighed according to the formula proportion into the clean glass container, add an appropriate amount of water, then add surfactant 5ml / L TritonX-100, adjust the pH to ph6-9 with concentrated hydrochloric acid, the dosage is about 5ml / L, then stir and filter, and then add distilled water to the obtained filtrate to a certain amount.

The method of using tops as chromogen substrate to detect FFA in serum or plasma has high accuracy and small measurement error. Among many Trinder's chromogens, it is the most suitable chromogen in the standards of small acetyl CoA, high stability and large molar absorption coefficient. Besides tops, the chromogen substrates produced by Desheng also include toos, Maos, ADPS, etc., which are suitable for the detection of blood glucose and other biochemical indexes.