Introduction and preparation method of biological buffer BICINE CAS NO.150-25-4

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng is a manufacturer of a series of biological buffer products, with a variety of products, which can provide you with more comprehensive services;

3. Desheng has been in this industry for 16 years. Our products are independently developed and manufactured. Biological buffer has high purity and guaranteed product quality.

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Buffer is a solution that can resist pH changes when a small amount of acid or alkali and water are added. The pH buffer system plays an important role in maintaining the normal pH value of organisms and the normal physiological environment. Most cells can only operate in a very narrow pH range. It needs to have a buffer system to resist the pH changes that occur during metabolism. There are three main pH buffer systems in the organism, they are protein and bicarbonate buffer systems. The amount of each buffer system It is different in various types of cells and organs.
In biochemical research work, buffer solutions are often used to maintain the pH of the experimental system. The pH value of the solution system in the research work often directly affects the effectiveness of our work. If the pH value of the extraction enzyme experimental system changes or changes too much , will reduce the enzyme activity or even completely inactivate. So we have to learn to prepare buffers.
Bicine, one of the commonly used biological buffers, is an amphoteric amino acid buffer that is active in the pH range of 7.6-9.0 (pKa of 8.3 at 25°C). Recommended buffer for low temperature biochemical work. Bicaine is used to prepare stable serum guanylinase substrate solutions. Methods have been published for protein separation using dihydropyridine in thin layer ion exchange chromatography. Dihydropyridine has been used for peptide and protein crystallization. Finished Buffer Standard
The preparation method of Bicine solution (about 1-2L):
(1) With 0.1M solution (A): Bicine16.317g/deionized water 1,000ml
(2) With 0.1M NaOH solution (B): NaOH 4g/deionized water 1,000ml
(3) pH5.11,000ml(A)+0ml(B)(A):(B)=5:0
pH7.81,000ml(A)+200ml(B)(A):(B)=5:1
pH8.21,000ml(A)+400ml(B)(A):(B)=5:2
pH8.61,000ml(A)+600ml(B)(A):(B)=5:3
pH10.41,000ml(A)+800ml(B)(A):(B)=5:4
Hubei Xindesheng Material Technology Co., Ltd. specializes in the production of various biological buffers, please inquire if necessary.